Kerusakan dinding sel Escherichia coli K1.1 oleh minyak atsiri temu kunci (Kaempferia pandurata) [Cell wall disruption of Escherichia coli Kdchia coli K1 1 by temu kunci (Kaeempferia pandurata) essential oil]
| Main Authors: | Miksusanti, Miksusanti, Betty Sri Laksmi Jennie, Betty Sri Laksmi Jennie, Bambang Ponco, Bambang Ponco, Gatot Tri Mulyadi, Gatot Tri Mulyadi |
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| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
Pusat Penelitian Biologi - LIPI
, 2008
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| Subjects: | |
| Online Access: |
http://eprints.unsri.ac.id/6218/2/file?file=pdf%252Fmetadata%2D121935.pdf http://eprints.unsri.ac.id/6218/ |
Daftar Isi:
- Antibacterial of temu kunci (Kaempferia padurata) essential oil against Escheria coli K1.1 cell wall and altering it. minimum inhibitory concentration (MIC) of temu kunci essential oil is 0.11% (v/v). further studies were conducted using the concentration of 1 MIC and 2 MIC. Leakage phenomena were monitored with atomic adsorption spectrometry (AAS), and ultraviolet spectrophotometry (UV). alteration of cell wall was analyzed with scanning electron microscopy (SEM). the optical density values observed by UV spectrophotometer for protein and nucleic acid leakage were 0.3813-0.6573 at 280 nm and 0.2186-0.5603 at 260 nm. the result showed that K. Pandurata essential oil could leak the inorganic ion Ca+2 17-53%, and K+1 9-43% from the bacteria and alter the cell wall of the bacteria