Endophytic fungi isolated from Sambiloto (Andrographis paniculata Nees) as a source of fungal lipid production
| Main Authors: | Elfita, Elfita, Muharni, Muharni, Munawar, Munawar |
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| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
India
, 2015
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| Subjects: | |
| Online Access: |
http://eprints.unsri.ac.id/5876/1/JCPR%2D2015%2D7%2D9S%2D66%2D69.pdf http://eprints.unsri.ac.id/5876/ |
Daftar Isi:
- Endophytic fungi isolated from sambiloto (Andographis paniculata), were investigated for fungal lipid production as a biodiesel precursors. The fungi Aspergillus sp1 (SbD3), Aspergillus sp2 (SbD5), Aspergillus sp3 (SbD6), and Penicillium sp (SbD4) were cultured in flasks with cultivated in Potato Dextrose Broth (PDB) medium for 8 days in static. Dried fungal biomass (30 g) were subjected to addition of each solvent in chloroform, methanol, water (1:2:1 (v/v/v). The reaction mixture was left to reflux for 24 h. After that period, the reaction mixture was filtered and concentrated in a rotatory evaporator. The lipid compounds were then extracted by liquid-liquid extraction using nhexane, and concentrated in a rotatory evaporator. The fungal lipid were determined on the basis of spectroscopic analysis (FTIR and 1H-NMR). In conclusion, this work revealed the possibility of using the promising Aspergillus sp2 (SbD5) in biodiesel production.