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Efek Asam Metoksiasetat (MAA) Terhadap Indeks Mitosis dan Blastosis Abnormal Mencit (Mus musculus) Swiss Webter

Main Authors: Riyanto, Riyanto, Sudarwati, Sri, Sumarsono, Sony Heru
Format: Article PeerReviewed application/pdf
Terbitan: STIK Siti Khadijah Plg , 2013
Subjects:
QL Zoology
Online Access: http://eprints.unsri.ac.id/5066/1/Efek_MAA_terhadap_IMdan_BA_Mencit__Jurnal_Kes_Multi_Science_STIK_Siti_Khadijah_Plg___SIPKD_.pdf
http://eprints.unsri.ac.id/5066/2/Efek_MAA_terhadap_IMdan_BA_Mencit__Jurnal_Kes_Multi_Science_STIK_Siti_Khadijah_Plg___SIPKD_.pdf
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ctrlnum 5066
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title>Efek Asam Metoksiasetat (MAA) Terhadap Indeks Mitosis dan Blastosis Abnormal Mencit (Mus musculus) Swiss Webter</title><creator>Riyanto, Riyanto</creator><creator>Sudarwati, Sri</creator><creator>Sumarsono, Sony Heru</creator><subject>QL Zoology</subject><description>The research about effects MAA on mitotic index and abnormal blastocysts of mice (Mus musculus) Swiss Webster have been studied. The purpose of this study was to determine the effect of MAA on mitotic index and abnormal blastocysts of mice (Mus musculus) Swiss Webter. Blastocyst stage mouse embryos were collected from superovulated mice with PMSG (Folligon) 5 IU / mice and hCG (Chorulon) 5 IU / mice. Treatment group mice 2 days of gestation were given MAA dose of 2.5 mmol / kg bw by gavage, whereas the control group was given only the solvent MAA. Collection performed on pregnant mice blastocysts 3.5 days killed by neck dislocation. Two hours before the mice were killed, mice were given kolkhisin 2 mg / kg bw intraperitoneally. Observations bermitosis cells and the number of constituent blastomeres derived from blastocysts disaggregation results. Mouse blastocysts mitotic index was calculated by counting the number of blastomeres were mitotic divided by total blastocyst blastomeres. The results showed, that the administration of MAA can lower mitotic index, number of blastomeres blastocyst, number of blastomeres were mitotic and number of metaphase phase significantly (p &lt;0.05) compared with the control group. In addition, MAA tend to increase the percentage of abnormal blastocysts as fragmentation blastocysts, blastomere degenerates, debris + zona pellucida, blastocysts without zona pellucida and unequal blastomeres size. It can be concluded that, MAA is cytotoxic for mouse blastocysts lower mitotic index significantly and tended to increase the percentage of abnormal blastocysts such as fragmentation blastocysts, blastomere degenerates, debris + zona pellucida, blastocysts without zona pellucida and unequal blastomeres size.</description><publisher>STIK Siti Khadijah Plg</publisher><date>2013-12-01</date><type>Journal:Article</type><type>PeerReview:PeerReviewed</type><type>File:application/pdf</type><identifier>http://eprints.unsri.ac.id/5066/1/Efek_MAA_terhadap_IMdan_BA_Mencit__Jurnal_Kes_Multi_Science_STIK_Siti_Khadijah_Plg___SIPKD_.pdf</identifier><type>File:application/pdf</type><identifier>http://eprints.unsri.ac.id/5066/2/Efek_MAA_terhadap_IMdan_BA_Mencit__Jurnal_Kes_Multi_Science_STIK_Siti_Khadijah_Plg___SIPKD_.pdf</identifier><relation>http:/www.unsri.ac.id</relation><identifier>Riyanto, Riyanto and Sudarwati, Sri and Sumarsono, Sony Heru (2013) Efek Asam Metoksiasetat (MAA) Terhadap Indeks Mitosis dan Blastosis Abnormal Mencit (Mus musculus) Swiss Webter. Jurnal Ilmiah Muti Science, 03 (01). pp. 50-57. ISSN 2087-4847</identifier><relation>http://eprints.unsri.ac.id/5066/</relation><recordID>5066</recordID></dc>
format Journal:Article
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PeerReview:PeerReviewed
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author Riyanto, Riyanto
Sudarwati, Sri
Sumarsono, Sony Heru
title Efek Asam Metoksiasetat (MAA) Terhadap Indeks Mitosis dan Blastosis Abnormal Mencit (Mus musculus) Swiss Webter
publisher STIK Siti Khadijah Plg
publishDate 2013
topic QL Zoology
url http://eprints.unsri.ac.id/5066/1/Efek_MAA_terhadap_IMdan_BA_Mencit__Jurnal_Kes_Multi_Science_STIK_Siti_Khadijah_Plg___SIPKD_.pdf
http://eprints.unsri.ac.id/5066/2/Efek_MAA_terhadap_IMdan_BA_Mencit__Jurnal_Kes_Multi_Science_STIK_Siti_Khadijah_Plg___SIPKD_.pdf
http:/www.unsri.ac.id
http://eprints.unsri.ac.id/5066/
contents The research about effects MAA on mitotic index and abnormal blastocysts of mice (Mus musculus) Swiss Webster have been studied. The purpose of this study was to determine the effect of MAA on mitotic index and abnormal blastocysts of mice (Mus musculus) Swiss Webter. Blastocyst stage mouse embryos were collected from superovulated mice with PMSG (Folligon) 5 IU / mice and hCG (Chorulon) 5 IU / mice. Treatment group mice 2 days of gestation were given MAA dose of 2.5 mmol / kg bw by gavage, whereas the control group was given only the solvent MAA. Collection performed on pregnant mice blastocysts 3.5 days killed by neck dislocation. Two hours before the mice were killed, mice were given kolkhisin 2 mg / kg bw intraperitoneally. Observations bermitosis cells and the number of constituent blastomeres derived from blastocysts disaggregation results. Mouse blastocysts mitotic index was calculated by counting the number of blastomeres were mitotic divided by total blastocyst blastomeres. The results showed, that the administration of MAA can lower mitotic index, number of blastomeres blastocyst, number of blastomeres were mitotic and number of metaphase phase significantly (p <0.05) compared with the control group. In addition, MAA tend to increase the percentage of abnormal blastocysts as fragmentation blastocysts, blastomere degenerates, debris + zona pellucida, blastocysts without zona pellucida and unequal blastomeres size. It can be concluded that, MAA is cytotoxic for mouse blastocysts lower mitotic index significantly and tended to increase the percentage of abnormal blastocysts such as fragmentation blastocysts, blastomere degenerates, debris + zona pellucida, blastocysts without zona pellucida and unequal blastomeres size.
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