Characterization of Chitosanase from Indralaya Swamp Bacteria, South Sumatera Indonesia
| Main Authors: | Baehaki, Ace, Herpandi, Herpandi |
|---|---|
| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
Chemic Publishing Co
, 2013
|
| Subjects: | |
| Online Access: |
http://eprints.unsri.ac.id/2375/1/Ace_Baehaki_%2D_Asian_J_of_biochemistry.PDF http://eprints.unsri.ac.id/2375/ |
Daftar Isi:
- The extracellular chitosanase-production capacity of 14 bacterial isolates taken from Indralaya Swamp South Sumatera, was evaluated in liquid cultures. The GB2 isolate was selected due to its high chitosanase activity. The optimum pH and temperature of chitosanase produced by GB2 were 8 and 300C, respectively. Ca2+ and Fe2+ increased GB2 chitosanase whereas Na+ and K+ inhibited the enzyme. Study on the effect of metals ion indicated that chitosanase GB2 was metaloenzyme. Moleculer weights were determined by using SDS-PAGE and zymogram technique. Moleculer weight of chitosanase isolate GB2 was 36 - 110 kD.