KERAGAMAN GENETIK POPULASI KAYU KUKU (Pericopsis mooniana, (THWAITES)THWAITES) DI HUTAN LAMEDAI BERDASARKAN PENANDA RAPD

Main Authors: Nurtjahjaningsih, ILG, Widyatmoko, AYPBC, Rimbawanto, Anto
Other Authors: Balai Besar Penelitian dan Pengembangan Bioteknologi dan Pemuliaan Tanaman Hutan
Format: Article info application/pdf Journal
Bahasa: eng
Terbitan: Center for Forest Biotechnology and Tree Improvement (CFBTI) , 2019
Subjects:
Online Access: http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5219
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5219/4741
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fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="id-ID">KERAGAMAN GENETIK POPULASI KAYU KUKU (Pericopsis mooniana, (THWAITES)THWAITES) DI HUTAN LAMEDAI BERDASARKAN PENANDA RAPD</title><creator>Nurtjahjaningsih, ILG</creator><creator>Widyatmoko, AYPBC</creator><creator>Rimbawanto, Anto</creator><subject lang="id-ID">natural reserve; genetic diversity; private allele; dendrogram; AMOVA</subject><description lang="id-ID">Vulnerable status of kayu kuku (Pericopsis mooniana) is due to heavily wood harvesting. Aim in this study is to asses genetic diversity and structure of kayu kuku at Lamedai forest, Southeast Sulawesi using RAPD markers. Leaves samples were collected from 4 natural populations at Lamedai forest i.e. Lamedai natural reserve,&#xA0;Lamedai village, Balijaya and Tangketada. The 22 polymorphic loci of 5 RAPD markers were subjected to the genetic analysis, binary data were calculated by GenAlex software to obtain parameters in genetic diversity within population and analysis molecular variant (AMOVA), while PopTree software was used to construct a dendrogram. Results showed that genetic diversity of the 4 populations was in moderate level (mean He: 0.361 &#xB1; 0.017) and the values was similar each other. There was no private allele at any populations, indicating that&#xA0;there was no genetic specification. A dendrogram analysis showed that Lamedai natural reserve genetically closed to Tangketada population and Balijaya, while Lamedai was separated into another cluster. AMOVA analysis showed 13% genetic diversity were observed among populations. In conclution, kayu kuku at Lamedai forest might be a large and continuous population in ancient period.</description><publisher lang="en-US">Center for Forest Biotechnology and Tree Improvement (CFBTI)</publisher><contributor lang="id-ID">Balai Besar Penelitian dan Pengembangan Bioteknologi dan Pemuliaan Tanaman Hutan</contributor><date>2019-06-30</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5219</identifier><identifier>10.20886/jpth.2019.13.1.25-32</identifier><source lang="en-US">Jurnal Pemuliaan Tanaman Hutan; Vol 13, No 1 (2019): Jurnal Pemuliaan Tanaman Hutan; 25-32</source><source>2527-8665</source><source>1693-7147</source><language>eng</language><relation>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5219/4741</relation><rights lang="en-US">Copyright (c) 2019 Jurnal Pemuliaan Tanaman Hutan</rights><rights lang="en-US">https://creativecommons.org/licenses/by-nc-sa/4.0</rights><recordID>--ejournal.forda-mof.org-ejournal-litbang-index.php-index-oai?verb=ListSets:article-5219</recordID></dc>
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author Nurtjahjaningsih, ILG
Widyatmoko, AYPBC
Rimbawanto, Anto
author2 Balai Besar Penelitian dan Pengembangan Bioteknologi dan Pemuliaan Tanaman Hutan
title KERAGAMAN GENETIK POPULASI KAYU KUKU (Pericopsis mooniana, (THWAITES)THWAITES) DI HUTAN LAMEDAI BERDASARKAN PENANDA RAPD
publisher Center for Forest Biotechnology and Tree Improvement (CFBTI)
publishDate 2019
topic natural reserve
genetic diversity
private allele
dendrogram
AMOVA
url http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5219
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5219/4741
contents Vulnerable status of kayu kuku (Pericopsis mooniana) is due to heavily wood harvesting. Aim in this study is to asses genetic diversity and structure of kayu kuku at Lamedai forest, Southeast Sulawesi using RAPD markers. Leaves samples were collected from 4 natural populations at Lamedai forest i.e. Lamedai natural reserve, Lamedai village, Balijaya and Tangketada. The 22 polymorphic loci of 5 RAPD markers were subjected to the genetic analysis, binary data were calculated by GenAlex software to obtain parameters in genetic diversity within population and analysis molecular variant (AMOVA), while PopTree software was used to construct a dendrogram. Results showed that genetic diversity of the 4 populations was in moderate level (mean He: 0.361 ± 0.017) and the values was similar each other. There was no private allele at any populations, indicating that there was no genetic specification. A dendrogram analysis showed that Lamedai natural reserve genetically closed to Tangketada population and Balijaya, while Lamedai was separated into another cluster. AMOVA analysis showed 13% genetic diversity were observed among populations. In conclution, kayu kuku at Lamedai forest might be a large and continuous population in ancient period.
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