Perbanyakan Lili (Lilium longiflorum Thunb.) Secara In Vitro dengan Zat Pengatur Tumbuh BAP dan NAA
| Main Authors: | Deswiniyanti, Ni Wayan, Lestari, Ni Kadek Dwipayani, Astarini, Ida Ayu, Hardini, Yunita |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Indonesian Society for Horticulture / Department of Agronomy and Horticulture
, 2020
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| Online Access: |
http://journal.ipb.ac.id/index.php/jhi/article/view/26421 http://journal.ipb.ac.id/index.php/jhi/article/view/26421/20948 |
Daftar Isi:
- Tanaman lili (Lilium longiflorum Thunb.) merupakan tanaman hias dan bunga potong. Permintaan bunga potong lili sangat menjanjikan tetapi ketersediaan bibit masih terbatas. Penelitian ini terdiri dari perkecambahan biji lili secara in vitro pada media 1⁄2 Murashige & Skoog (MS), multiplikasi bagian eksplan pangkal dan ujung tunas pada berbagai perlakuan media MS kombinasi ZPT BAP dan NAA. Tujuannya untuk mendapatkan kombinasi media yang tepat dan mengetahui persentase tertinggi bagian eksplan yang mampu tumbuh tunas, akar dan kalus. Kombinasi media MS dengan BAP dan NAA yaitu (a) kontrol 0:0 mg L-1, (b) 0:0.1 mg L-1, (c) 0:1 mg L-1, (d) 0.1:0 mg L-1, (e) 0.1:0.1 mg L-1, (f) 0.1:1 mg L-1, (g) 1:0 mg L-1, (h) 0.1:1 mg L-1, (i) 1;1 mg L-1. Penelitian ini disusun dalam RAL faktorial dua faktor yaitu kombinasi pemberian BAP dan NAA, multiplikasi eksplan bagian pangkal dan ujung tunas. Biji lili mampu berkecambah sebanyak 58%. Media c dan i yang mampu menginduksi eksplan untuk tumbuh tunas, akar dan kalus. Media c mampu menginduksi tunas, akar dan kalus, media i mampu menginduksi akar dan kalus. Persentase respon pertumbuhan eksplan bagian pangkal tunas yaitu pertumbuhan tunas 11.1% dan akar 13.3%, sedangkan eksplan bagian ujung tunas menunjukkan pertumbuhan akar 6.7% tanpa pertumbuhan tunas. Pertumbuhan kalus menunjukkan hasil yang sama pada eksplan pangkal dan ujung tunas yaitu 4.4%.
- Lily plant (Lilium longiflorum Thunb.) is an ornamental and cut flower. The demand for cut lily flowers has a promising demand, but the availability of seedling is llimited. This study consisted of in vitro germination of lily seeds on 1⁄2 Murashige & Skoog (MS) medium, multiplication of base and shoot-tip explants in various treatments of MS medium with the combination of plant growtht regulator (PGR) BAP and NAA. The aims of this research were to obtain the appropriate combination of media and the parts of explants of growing shoots, roots and callus.The combinations of MS medium with BAP and NAA are (a)control 0:0 mg L-1, (b)0:0.1 mg L-1, (c)0:1 mg L-1, (d)0.1:0 mg L-1, (e)0.1:0.1 mg L-1, (f)(0.1:1 mg L-1), (g)1:0 mg L-1, (h)0.1:1 mg L-1, (i)1:1 mg L-1. The research were arranged as factorial design with two factor in Randomized Complete Block Design (RCBD). The first factors were combination of BAP and NAA, and the second one was the types of explant (base part of shoot and shoot tip). Lily seeds were succesfully germinated as much as 58%. Medium c and i succesfully induced explants to grow buds, roots and callus. Medium c induced shoots, roots and callus, meanwhile medium i induced roots and callus. Percentage of response growth of shoot base explants were 11.1% shoot growth and 13.3% roots, while shoot tip explants only showed 6.7% root growth without shoot growth. The growth of callus showed the same results on the base and shoot tip explants (4.4%).