Expression of circulating miR-200c and vascular endothelial growth factor-A (VEGF-A) mRNA as potential biomarker in human hepatocellular carcinoma
| Main Authors: | Qoriansas, Nanda, Renovaldi, Dede, Raditya, Juwita, Lestari, Puji, Gumilas, Nur Signa, Suharno, ., Heriyanto, Didik Setyo, Ratnasari, Neneng, Mubarika H, Sofia |
|---|---|
| Other Authors: | LPDP (lembaga pengelola dana pendidikan), |
| Format: | Article info application/pdf Journal |
| Bahasa: | eng |
| Terbitan: |
Journal of the Medical Sciences (Berkala ilmu Kedokteran)
, 2019
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| Subjects: | |
| Online Access: |
https://jurnal.ugm.ac.id/bik/article/view/28560 https://jurnal.ugm.ac.id/bik/article/view/28560/pdf |
Daftar Isi:
- Hepatocellular carcinoma (HCC) is a common liver disease that causes significant publichealth problems throughout the world, including in Indonesia. The HCC is the six mostcommon cancers and second cancer-related deaths among men in the world. Recentlyit was reported that the microRNA is an important player in hepatocarcinogenesis. Theexpression of MiRNA-200c is often regulated in primary HCC and HCC cell lines. Vascularendothelial growth factor-A (VEGF-A) is a regulator of angiogenesis that has been reportedas miR-200c target gene. This study was conducted to measure expression levels in miR-200c and mRNAVEGF-A and their potential role as biomarkers at HCC. A total of 36HCC patients and 36 healthy subjects were included in this study. The relative expressionof miRNA-200c and mRNA VEGF-A was quantified using reverse transcription real timequantitative PCR (qRT PCR). Relative expression was calculated using . Unpaired t-testwas used to compare the expression levels of circulating miRNA-200c and mRNA VEGF-Ain HCC patients and healthy subjects. Pearson test was used to determine correlationbetween circulating miR-200c expression and mRNA VEGF-A expression levels. Theexpression levels of circulating miR-200c in HCC patients were lower compared to healthysubjects although it was not significant (p = 0.258). Conversely, the expression levelsof circulating mRNA VEGF-A in HCC patients were significantly higher compared tohealthy subjects (p = 0.001). The relative expression levels of circulating miR-200c werenegatively correlated with mRNA VEGF-A in HCC patients. In conclusion, the expressionlevels of mRNA VEGF-A in HCC patients are significantly deregulated in compared tothat in healthy subjects. Negative correlation between circulating miRN-200c and mRNAVEGF-A expression levels are reported in HCC patients.