Streptomyces spp. sebagai Pengendali Hayati Busuk Fusarium pada Bawang Merah: Streptomyces spp. as Biocontrol Agents of Fusarium Basal Rot on Shallots
| Main Authors: | Eka Wijayanti, Asih Nawangsih, Abdjad, Toding Tondok, Efi |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)
, 2024
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/jfiti/article/view/51263 http://journal.ipb.ac.id/index.php/jfiti/article/view/51263/27905 |
Daftar Isi:
- Streptomyces spp. as Biocontrol Agents of Fusarium Basal Rot on Shallots Streptomyces spp. have been widely studied as biological control agents of plant diseases because of their ability to produce various types of antibiotics, especially anti-fungal compounds. Its application to control Fusarium oxysporum f. sp. cepae causes fusarium basal rot disease on shallots in Indonesia has not been explored. The tests carried out included testing the effectiveness of Streptomyces spp. on shallot growth and fusarium basal rot disease incidence, as well as analyzing the compounds produced by Streptomyces spp. The test results show that Streptomyces spp. could not increase the growth parameters. Meanwhile, the application of Streptomyces spp. able to suppress the incidence of fusarium basal rot disease and prolong the incubation period of the pathogen. Analysis of the crude extract of S. lydicus ABF 59 showed that five dominant compounds are antifungal, namely, d-limonene, tridecane, o-cymene, 2,4-di-tert-butylphenol (2,4-DTBP), and hexadecanoic acid, methyl ester.
- Streptomyces spp. telah banyak diteliti sebagai agens pengendali hayati penyakit tanaman karena kemampuannya dalam menghasilkan berbagai jenis antibiotik, terutama senyawa-senyawa anticendawan. Aplikasinya untuk pengendalian Fusarium oxysporum f. sp. cepae penyebab penyakit busuk fusarium pada bawang merah di Indonesia belum dieksplorasi. Penelitian ini bertujuan mengevaluasi efektivitas Streptomyces spp. dalam mengendalikan F. oxysporum f. sp. cepae serta senyawa apa saja yang dihasilkan. Pengujian yang dilakukan meliputi uji efektivitas Streptomyces spp. terhadap pertumbuhan bawang merah dan insidensi penyakit busuk fusarium, serta analisis senyawa yang dihasilkan oleh Streptomyces spp. Hasil pengujian menunjukkan bahwa Streptomyces spp. tidak dapat meningkatkan parameter pertumbuhan. Sementara itu, pemberian Streptomyces spp. mampu menekan insidensi penyakit busuk fusarium dan memperpanjang masa inkubasi patogen. Analisis ekstrak kasar S. lydicus ABF 59 menunjukkan terdapat lima senyawa dominan yang bersifat anticendawan yaitu, d-limonene, tridecane, o-cymene, 2,4-di-tert-butylphenol (2,4-DTBP), dan hexadecanoic acid, methyl ester.