Viability and Inhibition of Endophytic Bacteria Pseudomonas fluorescens 4RS1 Against Phytophthora palmivora in Flour Formula
| Main Authors: | Khaeruni, Andi, Hariyani, Hisein, Waode Siti Anima, Satrah, Vit Neru, Wijayanto, Teguh, Sutariati, Gusti Ayu Kade, Taufik, Muhammad |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)
, 2022
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/jfiti/article/view/41946 http://journal.ipb.ac.id/index.php/jfiti/article/view/41946/24229 |
Daftar Isi:
- Viability and Inhibition of Endophytic Bacteria Pseudomonas fluorescens 4RS1 Against Phytophthora palmivora in Flour Formula Pseudomonas fluorescens 4RS1 is an endophytic bacterium that has good inhibition against Phytophthora palmivora, and it has the potential to be developed as a biological agent for black pod rot disease in cocoa. It is necessary to have a formulation that can maintain viability and inhibition and facilitate its use and application, to apply this bacterial agent at the farm level. This study aimed to determine the ability of talc and tapioca flour as the ingredients of formulations that have strong solubility and maintain the viability and inhibition of P. fluorescens 4RS1. The results showed that the use of tapioca flour as the basis for the formulation of P. fluorescens 4RS1 was better than talc because it had high solubility and low precipitation. The addition of nutrients in the form of brown sugar, peptone, and CMC in the tapioca flour formulation was able to maintain the viability and inhibition of P. fluorescens 4RS1 for 12 weeks of storage.
- Pseudomonas fluorescens 4RS1 merupakan bakteri endofit yang memiliki daya hambat kuat terhadap Phytophthora palmivora sehingga potensial dikembangkan sebagai agens hayati penyakit busuk buah pada kakao. Agar aplikasi agens hayati ini dapat dilakukan oleh petani maka diperlukan suatu formula yang mampu mempertahankan viabilitas dan daya hambatnya, serta memudahkan penggunaannya. Penelitian ini bertujuan menentukan kemampuan talk dan tepung tapioka sebagai bahan dasar formula yang dilengkapi bahan tambahan nutrisi supaya mampu mempertahankan viabilitas dan daya hambat P. fluorescens 4RS1. Hasil penelitian menunjukkan bahwa penggunaan tepung tapioka sebagai bahan dasar formula P. fluorescens 4RS1 lebih baik dibandingkan dengan talk karena memiliki daya larut yang tinggi dan endapan yang rendah. Penambahan nutrisi berupa gula merah, pepton, dan CMC dalam formula tepung tapioka mampu mempertahankan viabilitas dan daya hambat P. fluorescens 4RS1 selama 12 minggu masa penyimpanan.