Viability and Virulence of Fusarium oxysporum f. sp. zingiberi Isolates from Boyolali and Temanggung Preserved for 17 Years in Sterile Soils
| Main Authors: | Soesanto, Loekas, Fakhiroh, Zaqiatul, Suharti, Woro Sri |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)
, 2022
|
| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/jfiti/article/view/34517 http://journal.ipb.ac.id/index.php/jfiti/article/view/34517/24076 |
Daftar Isi:
- Fusarium oxysporum f. sp. zingiberi is a soil-borne plant pathogen causing rhizome rot on ginger. This pathogen can survive in the soil for several years without a host plant. This study aimed to examine the viability and virulence of 21 isolates of F. oxysporum f. sp. zingiberi after being preserved for 17 years in sterile soil. Fungal viability was determined by descriptive method, while the experiment using randomized block design was conducted to examine the virulence of fungal isolates. The treatments consisted of control, 21 isolates of F. oxysporum f. sp. zingiberi from Boyolali and Temanggung, each treatment was replicated three times. The variables consisted of colony colors and diameters, macroconidia and microconidia shapes, growth time, dry weight of mycelia, conidia density, incubation period, affected area, rhizome wet weight difference, and waste index. The results showed that all fungal isolates which were stored in sterile soil for 17 years still had the ability to grow well on PDA medium and fill up petri dishes in 11–36 days. Moreover, all the isolates caused infection and disease symptoms development in ginger rhizome var. Gajah. Less virulence isolate was characterized by a long incubation period (6–12 days after inoculation) and smaller affected area of the rhizome.
- Viabilitas dan Virulensi Fusarium oxysporum f. sp. zingiberi Asal Boyolali dan Temanggung Setelah Disimpan Tujuh Belas Tahun di Dalam Tanah Steril Fusarium oxysporum f. sp zingiberi merupakan cendawan penyebab busuk rimpang pada jahe. Patogen ini dapat bertahan di tanah selama bertahun-tahun tanpa tanaman inang. Penelitian bertujuan untuk menguji viabilitas dan virulensi 21 galur F. oxysporum f. sp. zingiberi setelah disimpan tujuh belas tahun di dalam tanah steril. Variabel yang diamati adalah warna dan diameter koloni, bentuk makrokonidium dan mikrokonidium, waktu pertumbuhan, berat kering miselium, kepadatan konidium, masa inkubasi, luas serangan pada rimpang, selisih bobot basah rimpang, dan indeks sampah. Hasil penelitian menunjukkan bahwa semua galur F. oxysporum f. sp. zingiberi asal Temanggung dan Boyolali yang disimpan di tanah steril selama 17 tahun mampu tumbuh baik pada medium PDA dan mengisi penuh cawan petri antara 11–36 hari. Selain itu, semua galur menyebabkan gejala penyakit pada rimpang jahe var. Gajah. Isolat dengan tingkat virulensi yang rendah ditandai oleh masa inkubasi yang panjang (6–12 hari setelah inokulasi) dan luas area rimpang terserang yang terkecil.