Salivary neutrophils isolation of severe early childhood caries patients with flow cytometry analysis using magnetic beads and CD177 marker
| Main Authors: | Luthfi, Muhammad, Kusumaningsih, Tuti |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en
, 2016
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| Subjects: | |
| Online Access: |
http://e-journal.unair.ac.id/MKG/article/view/2753 http://e-journal.unair.ac.id/MKG/article/view/2753/2196 |
Daftar Isi:
- Background: Neutrophils are the first line of defense, not only serving as he killer of microbes through phagocytosis process, in which reactive oxygen species (ROS) and anti-microbial peptides were released, but also regulating activation of immune response. CD177 is a tidylinositol glycosylphosphate glycoprotein with a molecular weight of 58- 64-kDa exclusively found on neutrophils, neutrophilic metamyelocytes, and mielosit. CD177 expression, a protein on the cell surface with an average size ranging from 45% to 65%, is only found on subpopulations of neutrophils. Purpose: This study aims to analyze the effects of salivary neutrophil isolation using magnetic beads and CD177 marker on S-ECC patients. Method: The study is an observational analytic research with cross sectional approach using flow cytometry analysis on the S-ECC patients and the caries-free children who were asked to use mouthwash, NaCl 1.5%. For the isolation of neutrophils, magnetic beads labeled with FITC funds and CD177+ marker were used. Result: There were 77.66% of salivary neutrophils expressing CD177+ markers, successfully isolated in the S-ECC patients, while in the caries-free children there were 63.67% of salivary neutrophils. Conclusion: In the S-ECC patients, there were 77.66% of salivary neutrophils expressing CD177markers, successfully isolated, while in the caries-free children there were 63.67% of salivary neutrophils.