A study of cytotoxicity and proliferation of Cosmos caudatus Kunth leaf extract in human gingival fibroblast culture
| Main Authors: | Shabrina, Zhafira Nur, Sumarta, Ni Putu Mira, Pramono, Coen |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en
, 2018
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| Subjects: | |
| Online Access: |
http://e-journal.unair.ac.id/MKG/article/view/10366 http://e-journal.unair.ac.id/MKG/article/view/10366/7165 |
| ctrlnum |
article-10366 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">A study of cytotoxicity and proliferation of Cosmos caudatus Kunth leaf extract in human gingival fibroblast culture</title><creator>Shabrina, Zhafira Nur</creator><creator>Sumarta, Ni Putu Mira</creator><creator>Pramono, Coen</creator><subject lang="en-US">Cosmos caudatus leaf extract; wound healing; human gingival fibroblasts culture</subject><description lang="en-US">Background: Post-extraction dental sockets clinically resolve within a period of 3-4 weeks. However, complete healing and bundling of gingival fibers may require several months. Medication is therefore required to accelerate the healing process. Cosmos caudatus (C. caudatus), a local plant with antioxidant properties and high calcium content, has the potential to promote wound healing while also reportedly capable of strengthening bone. Previous studies have demonstrated the effectiveness of C. caudatus as an alternative treatment for post-menopausal osteoporosis by investigating the dynamic and cellular parameters of bone histomorphometry. Purpose: The study aimed to examine the citotoxicity and proliferation of human gingival fibroblast cells culture after the application of C. caudatus extract. Methods: Cultures of human gingival fibroblast cells with 5x104 cell density were divided into two groups and placed in a 30-well culture dish. The control group contained human gingival fibroblast cell culture without extract, while the experimental group consisted of human gingival fibroblast cells culture with extract. The concentrations of extract were 1200 μg/ml, 600 μg/ml, 300 μg/ml, 150 μg/ml, and 75 μg/ml. A toxicity test was conducted and the optimum concentration evaluated using an MTT assay, while fibroblast numbers on were calculated days 1 and 2 by means of a hemocytometer. Research data was analyzed using a one-way ANOVA test. Results: No toxicity was found. The optimum concentration was 600 μg/ml and fibroblast proliferation was significantly higher in the experimental group compared to the control group, p=0.002 (P<0.05). Conclusion: C. caudatus leaf extract is non-toxic and increases the proliferation of human gingival fibroblast culture at an optimum concentration of 600 μg/ml.</description><publisher lang="en-US">Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en</publisher><contributor lang="en-US"/><date>2018-12-31</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://e-journal.unair.ac.id/MKG/article/view/10366</identifier><identifier>10.20473/j.djmkg.v51.i4.p179-184</identifier><source lang="en-US">Dental Journal (Majalah Kedokteran Gigi); Vol 51, No 4 (2018): December 2018; 179-184</source><source>2442-9740</source><source>1978-3728</source><language>eng</language><relation>http://e-journal.unair.ac.id/MKG/article/view/10366/7165</relation><rights lang="en-US">Copyright (c) 2018 Dental Journal (Majalah Kedokteran Gigi)</rights><rights lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</rights><recordID>article-10366</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:eJournal |
| author |
Shabrina, Zhafira Nur Sumarta, Ni Putu Mira Pramono, Coen |
| title |
A study of cytotoxicity and proliferation of Cosmos caudatus Kunth leaf extract in human gingival fibroblast culture |
| publisher |
Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en |
| publishDate |
2018 |
| topic |
Cosmos caudatus leaf extract wound healing human gingival fibroblasts culture |
| url |
http://e-journal.unair.ac.id/MKG/article/view/10366 http://e-journal.unair.ac.id/MKG/article/view/10366/7165 |
| contents |
Background: Post-extraction dental sockets clinically resolve within a period of 3-4 weeks. However, complete healing and bundling of gingival fibers may require several months. Medication is therefore required to accelerate the healing process. Cosmos caudatus (C. caudatus), a local plant with antioxidant properties and high calcium content, has the potential to promote wound healing while also reportedly capable of strengthening bone. Previous studies have demonstrated the effectiveness of C. caudatus as an alternative treatment for post-menopausal osteoporosis by investigating the dynamic and cellular parameters of bone histomorphometry. Purpose: The study aimed to examine the citotoxicity and proliferation of human gingival fibroblast cells culture after the application of C. caudatus extract. Methods: Cultures of human gingival fibroblast cells with 5x104 cell density were divided into two groups and placed in a 30-well culture dish. The control group contained human gingival fibroblast cell culture without extract, while the experimental group consisted of human gingival fibroblast cells culture with extract. The concentrations of extract were 1200 μg/ml, 600 μg/ml, 300 μg/ml, 150 μg/ml, and 75 μg/ml. A toxicity test was conducted and the optimum concentration evaluated using an MTT assay, while fibroblast numbers on were calculated days 1 and 2 by means of a hemocytometer. Research data was analyzed using a one-way ANOVA test. Results: No toxicity was found. The optimum concentration was 600 μg/ml and fibroblast proliferation was significantly higher in the experimental group compared to the control group, p=0.002 (P<0.05). Conclusion: C. caudatus leaf extract is non-toxic and increases the proliferation of human gingival fibroblast culture at an optimum concentration of 600 μg/ml. |
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Universitas Airlangga |
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33 |
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library:university library |
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Perpustakaan Universitas Airlangga |
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468 |
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Dental Journal (Majalah Kedokteran Gigi) |
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4286 |
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KOTA SURABAYA |
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JAWA TIMUR |
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IOS4286 |
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2019-05-07T06:14:06Z |
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2020-11-29T22:30:32Z |
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