Pengembangan Metode Griess dengan Reduktor Logam Seng Berlapis Tembaga untuk Penetapan Kadar Metabolit Oksida Nitrat dalam Serum
| Main Authors: | Armita Sari, Pande Made Nova, Warditiani, Ni Kadek, Astuti, Ni Made Widi, Wirasuta, I Made Agus Gelgel |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University
, 2021
|
| Online Access: |
https://ojs.unud.ac.id/index.php/jfu/article/view/65348 https://ojs.unud.ac.id/index.php/jfu/article/view/65348/40163 |
Daftar Isi:
- The recent research concerned in method development for determination of nitric oxide metabolites in serum using spectrophotometry visible based on Griess reaction. The reduction of nitrate to nitrite was performed using copper coated zinc. The method was optimized and validated. The determination of total nitrite in samples was started with deproteinization using ZnSO4 and NaOH. The supernatants were reducted using copper coated zinc, reacted with diazotization and coupling reagents, then incubated. The absorbance was measured using spectrophotometer visible. The optimum reductant mass of copper coated zinc is 1.5 g with reduction time 10 minutes, coefficient variation ? 5%. Linierity of analytical method gives a coefficient correlation, r > 0.995. Relative procedural standard deviation (Vx0) was ? 5%, precision ? 15%, and recovery 85-115%.
- Telah dilakukan pengembangan metode dan penetapan kadar metabolit oksida nitrat dalam serum dengan metode spektrofotometri sinar tampak. Pengembangan metode pengukuran kadar total nitrit dalam serum dilakukan dengan reaksi Griess yang diawali dengan reduksi nitrat menjadi nitrit dengan reduktor seng lapis tembaga. Metode dioptimasi dan divalidasi. Pengujian sampel diawali dengan deproteinisasi serum menggunakan ZnSO4 dan NaOH, kemudian supernatan direduksi dengan granul seng lapis tembaga,direaksikan dengan reagen pendiazo dan pengkopling, absorbansinya diukur dengan menggunakan spektrofotometer sinar tampak. Data yang dihasilkan diolah secara statitistik. Massa reduktor granul seng lapis tembaga yang optimal adalah 1,5 g dengan waktu reduksi 10 menit, koefisien variansi ? 5%. Koefisien korelasi > 0,995 dengan Vx0 ? 5%, keseksamaan ? 15%, dan kecermatan 85-115%. Kata Kunci: Griess, nitrat, nitrit, oksida nitrat, reduktor, serum