Aktivitas Antibakteri Ekstrak Etanol Daun Kapas (Gossypium barbadense L.) terhadap Staphylococcus epidermidis dan Propionibacterium acnes
| Main Authors: | khumaidi, akhmad, Nugrahani, Arsa Wahyu, Gunawan, Febriani |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University
, 2020
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| Online Access: |
https://ojs.unud.ac.id/index.php/jfu/article/view/57847 https://ojs.unud.ac.id/index.php/jfu/article/view/57847/35279 |
Daftar Isi:
- Gossypium barbadense L. is one of the plants that has the potential to be developed into an antibacterial agent. This study aims to determine the antibacterial ability of chemical compounds in ethanol extract of Gossypium barbadense L. leaves to Staphylococcus epidermidis and Propionibacterium acnes bacteria which are characterized by the formation of inhibitory zones. Extraction of Gossypium barbadense L. leaves was carried out by maceration using 96% ethanol. Antibacterial activity testing was carried out by agar diffusion method with a cylinder plate technique and the extract was made a concentration series. TLC bioautography and chromogenic reagents are used in the identification of groups of compounds that have antibacterial activity. The results showed that the ethanol extract of Gossypium barbadense L. leaves gave the greatest inhibition at 70% extract concentration ie 11.40 ± 0.32 mm against Propionibacterium acnes and 50% extract concentration against Staphylococcus epidermidis bacteria which was 12.28 ± 0.63 mm. The group of compounds that are thought to be responsible for antibacterial activity are flavonoids. Keywords: Antibacterial, Gossypium barbadense L. Leaves, Propionibacterium acnes, Staphylococcus epidermidis
- Kapas (Gossypium barbadense L.) merupakan salah satu tanaman yang berpotensi dapat dikembangkan menjadi agen antibakteri. Penelitian ini bertujuan untuk mengetahui kemampuan antibakteri senyawa kimia di dalam ekstrak etanol daun kapas terhadap bakteri Staphylococcus epidermidis dan Propionibacterium acnes yang ditandai dengan terbentuknya zona hambat. Ekstraksi daun kapas dilakukan secara maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar dengan teknik sumuran dan ekstrak uji dibuat seri konsentrasi. Kromatografi lapis tipis (KLT) bioautografi dan pereaksi kromogenik digunakan dalam identifikasi golongan senyawa yang mempunyai aktivitas antibakteri. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kapas dengan konsentrasi 50% memberikan daya hambat terbesar terhadap Staphylococcus epidermidis dengan diameter 12,28±0,63 mm sedangkan ekstrak etanol 70% memberikan aktivitas terbesar terhadap Propionibacterium acnes dengan diameter 11,40±0,32 mm. Golongan senyawa yang diduga bertanggung jawab terhadap aktivitas antibakteri yaitu golongan flavonoid. Kata kunci: Antibakteri, Daun Kapas (Gossypium barbadense L.), Propionibacterium acnes, Staphylococcus epidermidis