A REVISED METHOD FOR SUCKER STERILIZATION TO SUPPORT THE IN VITRO PROPAGATION OF SAGO PALM (Metroxylon sagu Rottb.)
| Main Authors: | Tajuddin, Teuku, ., Karyanti, Sukarnih, Tati, Haska, Nadirman |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Agency for the Assessment and Application of Technology (BPPT)
, 2014
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| Online Access: |
http://ejurnal.bppt.go.id/index.php/JBBI/article/view/548 http://ejurnal.bppt.go.id/index.php/JBBI/article/view/548/397 |
Daftar Isi:
- Hutan sagu (Metroxylon sagu Rottb.) dapat ditemukan dalam area yang cukup luas di wilayah Maluku dan Papua. Besarnya keanekaragaman hayati dari pohon sagu dapat dilihat di areal ini. Pohon sagu tumbuh secara alami terutama di daerah dataran atau rawa dengan sumber yang air melimpah. Tanaman sagu dapat diperbanyak dengan metode generatif melalui biji, dan vegetatif melalui tunas anakan. Dalam rangka mendukung perbanyakan pohon induk yang unggul secara in vitro dalam skala besar, perbaikan metode sterilisasi tunas anakan mutlak diperlukan. Tunas anakan muda (15-20 cm) yang diperoleh dari Propinsi Papua digunakan sebagai eksplan. Tujuan percobaan sterilisasi ini dilakukan untuk mendukung perbanyakan pohon sagu secara in vitro. Pada percobaan ini antibiotik digunakan untuk membersihkan jaringan internal eksplan dari jamur dan bakteri. Hasil percobaan ini menunjukkan bahwa campuran alkohol dan antibiotik dapat menekan pertumbuhan kontaminan.Kata kunci: Antibiotik, kontaminan jamur dan bakteri, kultur in vitro, metode sterilisasi, sagu ABSTRACTNatural sago (Metroxylon sagu Rottb.) forest can be found in large area in Maluku and Papua regions. There are wide genetic diversities of sago palm found in these areas. This palm grows along riverbanks and in swampy areas which are not suitable for other crops. Sago palm is propagated generatively by seed and vegetatively by suckers. With the purpose of establishing the in vitro culture method for a large-scale of mass clonally propagation of superior genotypes of sago palm, generating sterilized explants are very important. Young suckers (15-20 cm) obtained from areas of Papua Province were used as explants. The sterilization experiments were carrying out to support the tissue culture of sago palm. Sterilization was conducted using antibiotics in order to get rid of fungi and bacteria from inner part of explants tissues. The results showed that from all sterilization methods tested, the best result was treatment using alcohol and antibiotic as disinfectant agents.Keywords: Antibiotics, fungi and bacteria contaminants, in vitro culture, sterilization method, sago palm