The Effect of Histamine on Inward and Outward Currentsin Mouse Retinal Amacrine Cells
| Main Authors: | Horio, Kayo, Ohkuma, Mahito, Miyachi, Ei-ichi |
|---|---|
| Format: | Journal Book |
| Bahasa: | eng |
| Terbitan: |
Springer
, 2018
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| Subjects: | |
| Online Access: |
http://opac.unila.ac.id/ucs/index.php?p=show_detail&id=57545 |
Daftar Isi:
- Abstract The expression of H1 receptor has been reportedin amacrine cells of mouse and rat retinae. However, weassumed that other types of histamine receptors alsofunction in amacrine cells. In order to confirm that histaminemodulates the membrane potential in mouse amacrinecells, we measured voltage-gated currents usingwhole-cell configuration. Under voltage-clamp conditions,the amplitude of voltage-gated outward currents wasenhanced by the application of 100 lM histamine in 65%of amacrine cells. Histamine also increased the amplitudesof voltage-gated inward currents in 72% of amacrine cells.When antagonists of the histamine H1, H2, or H3 receptorswere applied to histamine-sensitive amacrine cells, allthree types of these inhibitors reduced the effect of histamine.Moreover, we classified recorded cells into seventypes based on their morphological characteristics. Two ofthe seven types, diffuse multistratified cells and AII amacrinecells, responded significantly to histamine. Theseresults indicate that histamine affected the membranepotential via three types of histamine receptors. Furthermore,there were differences in the responses to histamineamong types of amacrine cells. Histamine may be one ofthe important neurotransmitters and/or neuromodulators inthe visual processing.Keywords Retina Amacrine cell Histamine Patchclamptechnique Inward current Outward current