The PA?interacting host protein nucleolin acts as an antiviral factorduring highly pathogenic H5N1 avian influenza virus infection
| Main Author: | Gao, Zhao...[et al.] |
|---|---|
| Format: | Journal Book |
| Bahasa: | eng |
| Terbitan: |
Springer
, 2018
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| Subjects: | |
| Online Access: |
http://opac.unila.ac.id/ucs/index.php?p=show_detail&id=55843 |
Daftar Isi:
- AbstractPolymerase acidic (PA) protein is a multifunctional regulator of influenza A virus (IAV) replication and pathogenesis. In aprevious study, we reported that nucleolin (NCL) is a novel PA-interacting host protein. In this study, we further explored therole of NCL during highly pathogenic H5N1 avian influenza virus infection. We found that depletion of endogenous NCLin mammalian cells by siRNA targeting during H5N1 infection resulted in significantly increased viral polymerase activity,elevated viral mRNA, cRNA and vRNA synthesis, accelerated viral replication, and enhanced apoptosis and necrosis.Moreover, siRNA silencing of NCL significantly exacerbated the inflammatory response, resulting in increased secretion ofIL-6, TNF-?, TNF-?, CCL-4, CCL-8, IFN-?, IFN-? and IFN-?. Conversely, overexpression of NCL significantly decreasedIAV replication. Collectively, these data show that NCL acts as a novel potential antiviral factor during H5N1 infection.Further studies exploring the antiviral mechanisms of NCL may accelerate the development of new anti-influenza drugs.