In vitro Cytotoxicity and Antiâherpes Simplex Virus Type 1 Activity of Hydroethanolic Extract, Fractions, and Isolated Compounds from Stem Bark of Schinus terebinthifolius Raddi
| Main Authors: | Nocchi Samara Requena, Gislaine Franco de MouraâCosta, Novello Claudio Roberto, Rodrigues Juliana, Longhini Renata, Mello Joao Carlos Palazzo de, Filho Benedito Prado Dias, Nakamura Celso Vataru, Tania UedaâNakamura |
|---|---|
| Format: | Journal Book |
| Bahasa: | ind |
| Terbitan: |
Pharmacognosy Magazine
, 2016
|
| Subjects: | |
| Online Access: |
http://opac.unila.ac.id/ucs/index.php?p=show_detail&id=42848 |
Daftar Isi:
- ABSTRACTBackground: Herpes simplex virus type 1 (HSVâ1) is associated withorofacial infections and is transmitted by direct contact with infectedsecretions. Several efforts have been expended in the search fordrugs to the treatment for herpes. Schinus terebinthifolius is used inseveral illnesses and among them, for the topical treatment of skinwounds, especially wounds of mucous membranes, whether infectedor not. Objective: To evaluate the cytotoxicity and antiâHSVâ1 activityof the crude hydroethanolic extract (CHE) from the stem bark of S.terebinthifolius, as well as its fractions and isolated compounds.Materials and Methods: The CHE was subjected to bioguidedfractionation. The antiâHSVâ1 activity and the cytotoxicity of the CHE,its fractions, and isolated compounds were evaluated in vitro by SRBmethod. A preliminar investigation of the action of CHE in the virusâhostinteraction was conducted by the same assay. Results: CHE presentedflavanâ3âols and showed antiâHSVâ1 activity, better than its fractions andisolated compounds. The class of substances found in CHE can bind toproteins to form unstable complexes and enveloped viruses, as HSVâ1may be vulnerable to this action. Our results suggest that the CHEinterfered with virion envelope structures, masking viral receptors thatare necessary for adsorption or entry into host cells. Conclusion: Theplant investigated exhibited potential for future development treatmentagainst HSVâ1, but further tests are necessary, especially to elucidatethe mechanism of action of CHE, as well as preclinical and clinicalstudies to confirm its safety and efficacy.