UJI AKTIVITAS ANTIBIOTIK EKSTRAK ETANOL BIJI KOPI ROBUSTA (Coffea canephora L.) TERHADAP BAKTERI Staphylococcus aureus DAN Salmonella typhi
| Main Author: | Nursilri Meidania, 1618011046 |
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| Format: | Bachelors NonPeerReviewed Book Report |
| Terbitan: |
Fakultas Kedokteran
, 2020
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| Subjects: | |
| Online Access: |
http://digilib.unila.ac.id/64769/1/1.%20ABSTRAK%20%28INGGRIS%20%26%20INDONESIA%29.pdf http://digilib.unila.ac.id/64769/2/2.%20SKRIPSI%20FULL.pdf http://digilib.unila.ac.id/64769/3/3.%20SKRIPSI%20TANPA%20BAB%20PEMBAHASAN.pdf http://digilib.unila.ac.id/64769/ |
Daftar Isi:
- Background: Infectious diseases are one among the main causes of health problems with high morbidity and mortality rates. Excessive administration of antibiotics causes many bacteria to gain antibiotics resistance over time, one of them is gram-positive bacteria Staphylococcus aureus and gram-negative bacteria Salmonella typhi. It is necessary to use alternative therapies, for example through the use of herbal ingredients, one of which is coffee plant. The purpose of this study is to determine the antibiotic effect of robusta coffee bean (Coffea canephora L.) extract on Staphylococcus aureus and Salmonella typhi. Method: This study is a laboratory observational type with simple experimental research design (post-test only control group). The well diffusion method is used. The result is tested with Bonferroni Post Hoc test to see the average difference of each bacterium, while the Independent-Samples T Test are conducted to assess the significant difference between the two bacteria. Result: Based on the experiment performed on Staphylococcus aureus and Salmonella typhi, the average inhibition zone diameters are, in order, 18.83 mm and 16.33 mm respectively at 100% concentration, 13.42 mm and 9.08 mm respectively at 50% concentration, and 7.67 mm and 8.16 mm respectively at 25% concentration. According to One-Way Anova followed with Bonferroni Post Hoc test, there’s a significant difference in almost every treatment on each bacterium. While in Independent-Samples T Test, the significant difference between the bacteria was only found at 50% concentration application. Conclusion: It is presumed there’s antibiotic activity in the robusta coffee beans (Coffea canephora L.) extract against the Staphylococcus aureus and Salmonella typhi at concentrations 100%, 50%, and 25%. However, there is no real difference in the mean of the inhibition zones between the two bacterias except at 50%. Keywords: Antibiotic, robusta coffee bean, Salmonella typhi, Staphylococcus aureus, well diffusion method Latar Belakang: Penyakit infeksi termasuk penyebab masalah kesehatan utama dengan angka morbiditas dan kematian mortalitas tinggi. Pemberian antibiotik yang berlebih, seiring waktu mengakibatkan bakteri mengalami resistensi antibiotik, salah satu di antaranya yaitu bakteri gram positif Staphylococcus aureus dan bakteri gram negatif Salmonella typhi. Perlu dilakukan penggunaan terapi alternatif contohnya melalui penggunaan bahan-bahan herbal, salah satu tanaman yang berpotensi yaitu kopi. Tujuan penelitian ini mengetahui pengaruh aktivitas antibiotik ekstrak biji kopi robusta (Coffea canephora L.) terhadap bakteri Staphylococcus aureus dan Salmonella typhi. Metode: Penelitian ini bersifat observasional laboratorik dengan rancangan desain penelitian eksperimen sederhana (post-test only control group). Metode yang dilakukan yaitu metode difusi sumuran. Hasil penelitian diuji analisis Post Hoc untuk menguji perbedaan rata-rata terhadap tiap bakteri, sementara uji Independent-Samples T Test dilakukan untuk mengetahui apakah terdapat perbedaan rerata nyata antara kedua bakteri. Hasil: Berdasarkan perlakuan terhadap Staphylococcus aureus dan Salmonella typhi, didapatkan rerata diameter zona hambatnya secara berurut pada konsentrasi ekstrak biji kopi robusta 100% sebesar 18,83 dan 16,33 mm, 50% sebesar 13,42 dan 9,08 mm dan 25% sebesar 7,67 dan 8,16 mm. Hasil uji One-Way yang diikuti dengan Post Hoc Bonferroni, menunjukkan terdapat perbedaan rerata nyata pada hampir semua perlakuan. Sementara di uji Independent-Samples T Test perbedaan rerata yang nyata antara kedua bakteri hanya ditemukan pada konsentrasi 50%. Kesimpulan: Diduga terdapat aktivitas antibiotik pada ekstrak biji kopi robusta (Coffea canephora L.) terhadap bakteri Staphylococcus aureus dan Salmonella typhi pada konsentrasi 100%, 50% dan 25%. Namun, tidak terdapat perbedaan nyata pada rerata zona hambat antara kedua bakteri tersebut kecuali pada 50%. Kata kunci: Antibiotik, biji kopi robusta, metode difusi sumuran, Salmonella typhi, Staphylococcus aureus