EVALUASI SUPLEMENTASI FUKOIDAN Sargassum sp. DARI PERAIRAN LAMPUNG UNTUK RESPONS IMUN NON SPESIFIK UDANG VANAME (Litopenaeus vannamei) (Boone, 1931)
| Main Author: | HARSI DWI WIDYASTUTI, 1614111046 |
|---|---|
| Format: | Bachelors NonPeerReviewed Book Report |
| Terbitan: |
Fakultas Pertanian
, 2020
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| Subjects: | |
| Online Access: |
http://digilib.unila.ac.id/64468/1/1.%20ABSTRAK.pdf http://digilib.unila.ac.id/64468/2/2.%20SKRIPSI%20FULL.pdf http://digilib.unila.ac.id/64468/3/3.%20SKRIPSI%20TANPA%20BAB%20PEMBAHASAN.pdf http://digilib.unila.ac.id/64468/ |
Daftar Isi:
- Salah satu kendala dalam budidaya udang vaname adalah infeksi penyakit. Fukoidan terbukti memiliki aktivitas imunomodulator pada udang. Penelitian ini bertujuan untuk mengevaluasi respons imun non spesifik udang vaname (Litopenaus vannamei) yang diberi suplemen fukoidan Sargassum sp. dari perairan laut Lampung. Fukoidan diekstraksi menggunakan metode asam kemudian dikonfirmasi dengan FTIR spektroskopi. Ekstrak fukoidan dilapisi pada pakan dengan dosis 0, 750, dan 1500 mg/kg pakan. Fukoidan diberikan pada udang vaname (18 ± 0,4 g) sebanyak 3%, 3 kali sehari selama 14 hari. Hemolim diambil dari sinus ventral udang pada hari ke 0, 7, dan 14, untuk evaluasi respons imun yang meliputi jumlah total hemosit, aktivitas fagositosis, indeks fagositosis, total protein plasma dan profil histologi jaringan hepatopankreas diamati pasca perlakuan. Hasil penelitian menunjukkan bahwa suplementasi fukoidan mampu meningkatkan total hemocyte count (THC) udang mencapai 9,3 x 106 sel/ml, indeks fagositosis mecapai 2,8 dengan presentase aktivitas fagositosis (AF) sebesar 80%, dan total protein plasma mencapai 3,9 x 102 mg/ml. Tidak ada kerusakan pada jaringan hepatopankreas yang dipengaruhi oleh fukoidan baik pada dosis 750 mg/kg pakan maupun dosis 1500 mg/kg pakan. Penelitian ini diharapkan menjadi dasar pengembangan fukoidan dari Sargassum sp. di perairan laut Lampung sebagai imunostimulan dalam budidaya udang. Kata Kunci : Fukoidan, respons imun, Sargassum sp., udang vaname, perairan Lampung. One obstacle in the cultivation of white shrimp is disease infection. Fucoidan has been shown to have immunomodulatory activity in shrimp. This study aims to evaluate the non-specific immune response of pacific white shrimps (Litopenaus vannamei) which was given supplement fucoidan Sargassum sp. of Lampung seawater. Fucoidan was extracted using the acid method then confirmed by FTIR spectroscopy. Fucoidan extract was coated in feed at a doses of 0, 750, and 1500 mg / kg of feed. Fucoidan was given to white shrimp (18 ± 0.4 g) as much as 3%, 3 times a day for 14 days. Hemolymph was withdrawn from sinus ventral of shrimp on days days 0, 7, and 14, for evaluation of the immune response which includes total hemocyte count, phagocytic activity, phagocytic index, total protein plasma, and the histological profil of hepatopancreatic tissue was observed in post treatment. The results showed that fucoidan supplementation was able to increase the total hemocyte count (THC) of shrimp to reach 9,3x106 cells/ml, phagocytosis index reaches 2.8 with a percentage of phagocytosis activity (AF) of 80%, and total plasma protein reaches 3,9x102 mg/ml. There was no damage to the hepatopancreatic tissue that was affected by fucoidan, both at a dose of 750 mg/ kg of feed and a dose of 1500 mg/kg of feed. This research is expected to be the basis for the development of fucoidan from Sargassum sp. in Lampung seawater as an immunostimulant in shrimp culture. Keywords: Fucoidan, immune response, Sargassum sp., white shrimp, Lampung seawater.