STUDI BIODEGRADASI SELULOSA DARI LIMBAH PERTANIAN JERAMI PADI, ONGGOK, BONGGOL JAGUNG, DAN AMPAS NANAS OLEH TIGA FUNGI ISOLAT LOKAL
| Main Author: | HELDA, 1627011007 |
|---|---|
| Format: | Masters NonPeerReviewed Book Report |
| Terbitan: |
, 2020
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| Subjects: | |
| Online Access: |
http://digilib.unila.ac.id/63490/1/ABSTRAK.pdf http://digilib.unila.ac.id/63490/2/TESIS%20FULL.pdf http://digilib.unila.ac.id/63490/3/TESIS%20TANPA%20BAB%20PEMBAHASAN.pdf http://digilib.unila.ac.id/63490/ |
Daftar Isi:
- Pada penelitian ini telah dilakukan penelitian biodegradasi selulosa limbah pertanian jerami padi, onggok, bonggol jagung, dan ampas nanas oleh tiga fungi isolat lokal hasil dari isolasi. Penelitian ini bertujuan mengetahui kemampuan biodegradasi isolat lokal E-2-1, S-5-19, dan S-5-24 dalam membiodegradasi selulosa limbah pertanian tersebut baik sebagai isolat tunggal maupun konsorsium. Produk fermentasi diharapkan bermanfaat untuk meningkatkan kecernaan nutrisi pakan ternak. Tahapan penelitian ini meliputi proses peremajaan isolat, penyiapan media cair, inokulum, penumbuhan isolat, aktivitas selulolitik, biodegradasi oleh isolat dan fermentasi. Hasil penelitian menunjukkan bahwa pertumbuhan sel dalam OD (optical density) panjang gelombang 600 nm isolat E-2-1 sebesar 3,00 pada inkubasi 48 jam sebagai isolat terbaik. Sedangkan isolat S-5-19, dan Isolat S-5-24 sebesar 2,23 dan 2,81 pada inkubasi 36 jam. Aktivitas enzim isolat E-2-1 sebesar 2,50 U/mL pada inkubasi pada 48 jam, untuk isolat E-5-19 dan isolat E-5-19 sebesar 2,33 U/mL dan sebesar 2,22 U/mL pada inkubasi 36 jam. Biodegradasi selanjutnya oleh isolat tunggal ke tiga isolat tersebut waktu 6 hari terhadap jerami padi sebesar 35,0%, onggok sebesar 24,1%, bonggol jagung sebesar 25,0%, dan ampas nanas sebesar 26,6%. Biodegradasi konsorsium ke tiga isolat tersebut waktu 6 hari terhadap substrat jerami padi sebesar 40,1%, onggok sebesar 42,2%, bonggol jagung sebesar 41,5%, dan ampas nanas sebesar 43,9%, dengan demikian biodegradasi oleh konsorsium isolat lebih baik daripada isolat tunggal. Kata kunci : Biodegradasi, selulosa, isolat lokal, OD (optical density), aktivitas unit, dan fermentasi. abstract In this research, biodegradation research has been carried out on cellulose agricultural waste of rice straw, heaf, cornlump, and pineapple sediment by three fungi of local isolates resulting from isolation. This study aims to determine the ability of biodegradation of local isolates E-2-1, S-5-19, and S-5-24 in biodegradation of agricultural agricultural cellulose both as a single isolate and a consortium. Fermented products are expected to be useful for improving the digestibility of animal feed nutrition. The stages of this research include the process of rejuvenation of isolates, preparation of liquid media, inoculums, isolation growth, cellulolytic activity, biodegradation by isolates and fermentation. The results showed that cell growth in OD (optical density) wavelength of 600 nm isolates E-2-1 was 3,00 at 48 hours incubation as the best isolate. Whereas isolates S-5-19, and S-5-24 isolates were 2,23 and 2,81 at 36 hours incubation. The enzyme activity of isolate E-2-1 was 2,50 U / mL at incubation at 48 hours, for the isolate E-5-19 and E-5-19 isolates were 2,33 U / mL and 2.22 U / mL at 36 hours incubation. Biodegradation was then carried out by a single isolate to the three isolates within 6 days of rice straw at 35,0%, heaf at 24,1%, cornlump at 25,0%, and pineapple sediment at 26,6%. Biodegradation of the three isolates within 6 days of rice straw substrate was 40,1%, heaf was 42,2%, cornlump were 41,5%, and pineapple sediment was 43,9%, thus biodegradation by consortium was more better than a single isolate. Keywords: Biodegradation, cellulose, local isolates, OD (optical density), unit activity, and fermentation.