EFEK EKSTRAK METANOL MAKROALGA MERAH (Gracilaria sp) MAKROALGA COKELAT (Padina sp) DAN TAURIN TERHADAP KADAR GLUKOSA DARAH, KOLESTEROL TOTAL, JUMLAH DAN VIABILITAS SPERMATOZOA MENCIT (Mus musculus L.) YANG DIINDUKSI ALOKSAN
| Main Author: | Yoharnes, 1727021003 |
|---|---|
| Format: | Masters NonPeerReviewed Book Report |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
http://digilib.unila.ac.id/56680/1/ABSTRAK.pdf http://digilib.unila.ac.id/56680/2/TESIS%20FULL.pdf http://digilib.unila.ac.id/56680/2/TESIS%20TANPA%20BAB%20PEMBAHASAN.pdf http://digilib.unila.ac.id/56680/ |
Daftar Isi:
- Data pada tahun 2017, Indonesia memiliki 10,58 juta penderita diabetes dan menduduki jumlah ke dua terbanyak di kawasan Pasifik Barat dengan rentang usia sekitar 18-99 tahun. Kematian penderita diabetes tertinggi disebabkan oleh hiperglikemia yang ditandai dengan naiknya kadar gula darah. Gracilaria sp dan Padina sp adalah jenis rumput laut yang mengandung senyawa kimia yang berpotensi sebagai antihiperglikemia. Penelitian ini bertujuan mengetahui efek ekstrak metanol Gracilaria sp, Padina sp dan taurin terhadap kadar gula darah, kolesterol total, jumlah dan viabilitas spermatozoa mencit jantan yang diinduksi aloksan. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 5 (lima) kelompok perlakuan yaitu : K1 (kontrol negatif), K2 (kontrol positif) diinduksi aloksan, K3 (diinduksi aloksan dan diberi taurin dengan dosis 15,6 mg/bb/hari selama 14 hari), K4 (diinduksi aloksan dan diberi ekstrak Gracilaria sp dengan dosis 8 mg/bb/hari selama 14 hari), dan K5 (diinduksi aloksan dan diberi ekstrak Padina sp dengan dosis 8 mg/bb/hari selama 14 hari). Parameter yang diamati yaitu kadar glukosa darah, kolesterol total, jumlah spermatozoa, viabilitas spermatozoa, rerata berat badan, rerata berat organ hepar, rerata berat testis, histopatologi hepar, histopatologi pankreas, dan histopatologi tubulus seminiferus. Data dianalisis dengan One Way Anova dan dilanjutkan dengan uji Fisher pada taraf α =0,05. Hasil penelitian menunjukkan bahwa ekstrak metanol Gracilaria sp, Padina sp dan taurin mampu menurunkan kadar glukosa darah, kolesterol total, memperbaiki jumlah dan viabilitas spermatozoa pada mencit secara signifikan serta mampu memperbaiki jaringan hepar, memberikan efek protektif terhadap organ pankreas dan mampu memperbaiki tubulus seminiferus mencit yang diinduksi aloksan. Kata kunci : Diabetes, Gracilaria sp, M. musculus L, Padina sp, viabilitas. ABSTRACT Data in 2017, Indonesia has 10.58 million people with diabetes and occupies the second largest number in the Western Pacific region with an age range of around 18-99 years.The highest mortality of diabetics is caused by hyperglycemia which is characterized by an increase in blood sugar levels. Gracilaria sp and Padina sp are types of seaweed that contain chemical compounds that have the potential to be antihyperglycemic. This study aims to determine the effect of methanol extract of Gracilaria sp, Padina sp and taurine on blood sugar levels, total cholesterol, amount and viability of spermatozoa in male mice induced by alloxan. The research method used Completely Randomized Design (CRD) with 5 (five) treatment groups, namely: K1 (negative control), K2 (positive control) induced by alloxan, K3 (alloxan induced and given taurine at a dose of 15.6 mg / bb / day during 14 days), K4 (induced by alloxan and given Gracilaria sp extract at a dose of 8 mg / bb / day for 14 days), and K5 (induced by alloxan and given Padina sp extract at a dose of 8 mg / bb / day for 14 days). The parameters observed were blood glucose levels, total cholesterol, number of spermatozoa, viability of spermatozoa, average body weight, mean liver weight, mean testicular weight, liver histopathology, pancreatic histopathology, and histopathology of seminiferous tubules. Data were analyzed by One Way Anova and continued with the Fisher test at the level of α = 0.05. The results showed that the methanol extract of Gracilaria sp, Padina sp and taurine was able to reduce blood glucose levels, total cholesterol, improve the number and viability of spermatozoa in mice significantly and be able to repair liver tissue, provide a protective effect on the pancreatic organs and repair the seminiferous tubules of mice induced alloxan. Keywords: Diabetes, Gracilaria sp, M. musculus L, Padina sp, viability.