Collection and Identification of N-Fixing Bacteria on Location of Edamame Soybean Plants (Glycine max (L.) Merr.) in Jember District
| Main Authors: | Fajrin, Vivin Nuraini, Erdiansyah, Iqbal, Damanhuri, FNU |
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| Format: | Article info application/pdf Journal |
| Bahasa: | ind |
| Terbitan: |
Politeknik Negeri Jember
, 2017
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| Subjects: | |
| Online Access: |
https://agriprima.polije.ac.id/index.php/journal/article/view/v1i2-f https://agriprima.polije.ac.id/index.php/journal/article/view/v1i2-f/pdf |
Daftar Isi:
- This research purposed to known fixing bacteria at edamame soybean’s field Center in Jember Regency. This research was conducted along 3 months from September 2016 until November 2016. The depth of a land activity had done in Jember subdistrict and the testing of N Fixing Bacteria that had done in Biosains Laboratory from State Polytechnic of Jember. There were 2 factors with 6 treatments and 3 replications. The first factor was location factor (L) consisted of located in Jenggawah subdistrict (L1) and in Ajung subdistrict (L2) and the second factor was 3 levels of the depth land (k) consisted of 5 cm the depth of a land from a land surface (K1), 10 cm from a land surface (K2), 15cm from a land surface (K3). The data of this research analyzed as quantitative and qualitative. The quantitative data collected by counting the colony by TPC method (Total Plate Count) and the qualitative data had gotten from observation of gram staining, observation of the speed of growing bacteria and observation of a pure bacteria. The result of this research showed that bacteria which had isolated from vegetable soybean (Glycine max (L.) Merr.) was Rhizobium spp, it is bacteria type of N fixing bacteria, the result showed from observation of a pure bacteria was a pure white and pink after bred in Yema selection media+congo red.Rhizobium sp. which has the highest adaptation level is from Ajung District.It was taken at 15 cm below the ground surface. The adaptation levels 6,88x1030 CFU/gram of soil was a pure white and pink after bred in Yema selection media+congo red.
- Penelitian ini bertujuan untuk mengetahui dan mengidentifikasi bakteri penambat N pada pusat lokasi tanaman kedelai edamame (Glycine max (L.) Merr.) di Kabupaten Jember. Penelitian ini dilakukan dari bulan September 2016 hingga November 2016. Kegiatan penelitian dilakukan di Laboratorium Biosains Politeknik Negeri Jember. Penelitian ini terdiri dari 2 faktor dengan 6 perlakuan dan 3 ulangan, yaitu faktor Lokasi (L) yang terdiri dari lokasi pengambilan tanah di Kecamatan Jenggawah (L1) dan di Kecamatan Ajung (L2), faktor Kedalaman (K) terdapat 3 taraf yaitu kedalaman pengambilan tanah 5 cm dari permukaan tanah (K1), 10 cm dari permukaan tanah (K2), dan 15 cm dari permukaan tanah (K3). Data dianalisis secara kuantitatif dan kualitatif, data kuantitatif dilakukan dengan cara menghitung jumlah koloni bakteri menggunakan metode TPC (Total Plate Count), data kualitatif didapatkan dari pengamatan gram staining, pengamatan kecepatan pertumbuhan bakteri dan pengamatan kemurnian bakteri. Hasil penelitian ini menunjukan bahwa bakteri yang diisolasi dapat dikoleksi dan diidentifikasi sebagai jenis bakteri penambat N tipe bakteri Rhizobium spp. yang ditunjukkan dengan warna putih bening dan merah muda setelah dibiakkan dalam media seleksi YEMA + Congo Red. Bakteri Rhizobium sp. yang memiliki tingkat adaptasi tertinggi berasal dari Kecamatan Ajung pada kedalaman 15 cm dari permukaan tanah, yaitu sebesar 6,88x1030 CFU/g tanah.