Shoot Induction of Tobacco (Nicotiana tabacum L.) Var. Kasturi 2 With Various Concentrations of BAP Under in Vitro Condition
| Main Authors: | Anindiyati, Irma, Erawati, Dyah Nuning |
|---|---|
| Format: | Article info application/pdf Journal |
| Bahasa: | ind |
| Terbitan: |
Politeknik Negeri Jember
, 2020
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| Subjects: | |
| Online Access: |
https://agriprima.polije.ac.id/index.php/journal/article/view/v4i1-c https://agriprima.polije.ac.id/index.php/journal/article/view/v4i1-c/pdf |
Daftar Isi:
- Tobacco var. Kasturi 2 is typically tobacco of Jember that has high productivity. Generative propagation tends to produce less resembling seedling. Further development by Tissue culture is needed to obtained more seeds. Addition of plant growth promoter is one of factor that affects the success of in vitro propagation. This research aims to determine the effect of concentration of BAP on shoot induction of Tobacco var. Kasturi 2. This research was conducted in September 2018 to December 2018 at Tissue Culture Laboratory of State Polytechnic of Jember. This research was using Completely Randomized Design non factorial with 6 treatments. The treatment was addition of BAP with concentration of 0 ppm, 1 ppm, 2 ppm, 3 ppm, 4 ppm, and 5 ppm. The results of this research showed that the addition of 2 ppm BAP was the best treatment on shoot induction of Tobacco Kasturi 2 Variety with early budding of 12,17 day after inoculation, 100% explant produced buds, the average number of bud was 47,5 per explant and the average height of bud was 1,42 cm.
- Tembakau Kasturi 2 merupakan tembakau khas Jember yang memiliki produktifitas relatif tinggi. Perbanyakan secara generatif cenderung menghasilkan bibit yang kurang seragam sehingga dikembangkan dengan teknik kultur jaringan. Dalam kultur jaringan, penambahan zat pengatur tumbuh memberikan pengaruh yang nyata.Penelitian ini bertujuan untuk mengetahui pengaruh penambahan konsentrasi ZPT BAP terhadap induksi tunas tembakau varietas Kasturi 2. Penelitian ini dilaksanakan pada bulan September 2018 hingga Desember 2018 di Laboratorium Kultur Jaringan Politeknik Negeri Jember. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) Non Faktorial dengan 6 perlakuan yaitu penambahan BAP dengan konsentrasi 0 ppm, 1 ppm, 2 ppm, 3 ppm, 4 ppm, dan 5 ppm. Hasil penelitian menunjukkan bahwa penambahan BAP 2 ppm merupakan konsentrasi terbaik untuk induksi tunas tembakau Kasturi 2 dengan kedinian bertunas 12,17 hari setelah inokulasi, eksplan bertunas 100%, rerata jumlah tunas 47,50 buah per eksplan dan rerata tinggi tunas 1,42 cm.