PENGARUH PAPARAN ASAP ROKOK ELEKTRONIK (e- cigarette) DAN ROKOK KONVENSIONAL TERHADAP JUMLAH SEL LEYDIG PADA GAMBARAN HISTOLOGIS TESTIS TIKUS PUTIH (Rattus norvegicus) JANTAN STRAIN WISTAR
Daftar Isi:
- Background: In Indonesia the highest level of awareness about the existence of electronic cigarettes is in the community at the tertiary level by 29.4% and those in urban areas by 15.3%. Previous studies have shown other harmful substances in electronic cigarettes that are the same as conventional cigarettes which are thought to reduce the number of leydig cells in the testes. Previous studies have shown that there are harmful substances in e-cigarette which also found in the conventional ones. This leads to the increasing amount of leydig cells in the testicular. Objective: Finding the effect of e-cigarette and conventional cigarette to the amount of leydig cells in the testicular in male white rats (Rattus norvegicus strain wistar). Methods: Experimental research with post test control only group design. 16 rats were divided into 4 groups: normal group, conventional cigarettes with 2.3 mg nicotine, e-cigarettes with 3 mg nicotine, and e-cigarettes with 0 mg nicotine. The trachea was taken to provide histologic preparation with HE staining and observed with light microscope (400x). Data was analysed using One-way ANOVA and Post-Hoc test. Results: One-way ANOVA test showed significant difference (p = 0,001) among groups, the Post-Hoc test showed significant differences (p = 0,001) between normal group and electronic cigarette with 3mg nicotine and (p = 0,003) between convensional cigarettes and there was no significant differences among e-cigarette with 3mg nicotine and conventional cigarettes (p = 1,000). Conclusions: Smoke exposure of electronic cigarette (e-cigarette) with 3mg nicotine and conventional cigarettes have the same effect on decreasing the amount of leydig cells in the rats testicular.