Molecular and Histochemical Analysis of Jatropha curcas Linn Transgenic Using Tolerance Antibiotics Hygromycin (hpt) and B-Glucuronidase (gus-A) Gene
| Main Authors: | Maftuchah, Maftuchah, Zainudin, Agus, Pahlevi, Robby Wahyu, Anggriawan, Arik |
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| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Elsevier Ltd
, 2015
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| Subjects: | |
| Online Access: |
http://eprints.umm.ac.id/57864/7/Maftuchah%20Zainudin%20Pahlevi%20Anggriawan%20-%20Gus-A%20Gene%20Histochemicals%20Test%20Hpt%20Gene%20Jatropha%20Curcas%20Linn.pdf http://eprints.umm.ac.id/57864/ https://www.sciencedirect.com/science/article/pii/S1876610215000211 |
Daftar Isi:
- To improve Jatropha curcas's trait, genetic engineering technology genetic engineering development is needed. This study was aimed to analyze transgenic of J. curcas with plasmid vectors pCambia-1304. The study was conducted using primer hpt (forward) 5’-GATGCCTCCGCTCGAAGTAGCG-3’, (reverse) 5’-GCATCTCCCGCCGTGCAC-3’, gus-A (forward) 5’–GCCATTTGAAGCCGATGTCACGCC-3’ and (reverse) 5’-GTATCGGTGTGAGCGTCGCAGAAC-3’. The PCR results elucidated five plants (16.67%) positive hpt gene, 22 plants (77.33%) positive gus-A gene. Histochemical test results showed 16 plants (53.33%) positive hpt test. A total of 24 plants (80%) resulted positive gus-A gene on leaf and 11 plants (36.67%) resulted positive gus-A gene on the root.