Isolasi Dan Seleksi Isolat Bradyrhizobium japonicum Pada Nodel Tanaman Kedelai (Glycine max. (L) Merill) Yang Di Tanam DiBawah Tegakan Sengon
| Main Author: | Lestari Fatmawati, Sri |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
, 2008
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| Subjects: | |
| Online Access: |
http://eprints.umm.ac.id/5269/1/Isolasi_Dan_Seleksi_Isolat_Bradyrhizobium_japonicum.pdf http://eprints.umm.ac.id/5269/ |
Daftar Isi:
- Soya bean is also known as green manure because it can increase soil fertility level. Nowadays, agricultural fields in Indonesia has drastically lowered in number to become housing and accomodate industrial purposes. Soya bean planting using agroforestry system is considered to overcome such problems in Indonesia as well as an effort to optimize under-tree spaces (forest vegetations). Soya bean rooting system is capable of forming nodulation in which Rhizobium japonicum bacteria colonize. Rhizobium bacteria establish such beneficial symbiosis with soya bean roots to fixate free nitrogen (N2) from air (Rahmat, 1995). Rhizobium japonicum is employed as an effort to increase the number of soil-living rhizobium bacteria which in turn provide the plant with Nitrogen and thus increase the soya bean production (Freire, et. al. 1977). Bradyrhizobium japonicum inoculum on soya bean is necessarily needed if soil nitrogen is the limiting factor of plant growth (Idiyah, 1997). Rhizobium infection and colonization into plant roots and initial root nodules are fully influenced by Ca. Ca is needed in rhizobium infection on interface level with medium of soil (Yutono, 1985). This research is conducted to isolate, select, and describe Bradyrhizobium japonicum in soya bean grown under sengon tree. The research was conducted in trial field of Muhammadiyah University of Malang in Tegalgondo and Biotechnology laboratory of Muhammadiyah University of Malang starting from April up to October 2007. Stages of operation include field preparation, soya bean seed planting, nodules picking up, nodules sterilization, Bradyrhizobium japonicum isolation, keeping of isolated strains, Gram Test, and bacteria portrayal. The research resulted in that by isolating, selecting, and describing 7 bacteria strains were found on the basis of its growth speed and share same characters including varyingly colony colors, from yellow to brownish white with colony tip is branched round to fully round and share the same thickness (Thick).