PERBANDINGAN AKTIVITAS ANTIOKSIDANPADA APEL MANALAGI (Malus sylvestris Mill) DAN PRODUK OLAHAN DALAM BENTUK CUKA APEL DENGAN MENGGUNAKAN METODE ABTS DENGAN SPEKTROFOTOMETER UV-VIS
Daftar Isi:
- Background: Antioxidants are the chemical compounds that can contribute one or more electrons to the free radicals for slowing free radical reactions. One kind of plant that has potential as an antioxidant is manalagi apple (Malus sylvestris Mill). Manalagi apple and apple vinegar were used as the test samples. The testing process were through washing, crushing, pressing, filtering, adding bacteria, fermentation stage 1 and 2, and packaging stage. Based on some bibliography, there are antioxidant activity on apples. ABTS solution is one of the reagents that is used for antioxidant test that can clearly present free radicals with a UV-Vis spectrophotometer. The value of IC50 shows the level of free radical inhibition proxies against antioxidants. Objective: This study aimed to identify the comparing ratio of antioxidant activity between fresh manalagi apple and apple vinegar using ABTS method (2,2-Azinobis (3-ethylbenzothiazolin-6-sulphonic acid) Method: This study applied ABTS method which is one the method to measure antioxidant activity in natural materials using UV-Vis spectrophotometer. Results and Conclusions: The results showed the IC50 value from fresh manalagi apple is 52.602,9773 ppm and IC50 value from apple vinegar is 2.014.021,736 ppm, it showed that fresh apple has higher antioxidant activity compared with apple vinegar.