Effect of Extract from Pericarp of Mangosteen (Garcinia Mangostana Linn) as Anti-Inflammatory Agent in Rat Models with Atherosclerosis
Daftar Isi:
- Background. Atherosclerosis is an inflammatory disease characterized by intense immunological activity, which immune responses participate in every phase of atherosclerosis. On fundamental importance in atherogenesis is nuclear factor-κB (NF- κB) that regulates activation of adhesion molecules (ICAM-1) and secretion of pro-inflammatory cytokines such as tumor necrosis factor (TNF-α) interleukin-1 (IL-1), and interleukin-6 (IL-6). Aims. The extract from pericarp of mangosteen (Garcinia mangostana Linn ) were carried out to investigate for anti-inflammatory activity in rat models with atherosclerosis. Methods. The subjects of the study were 30 albino rats of (Rattus novergicus Strain wistar), divided into 5 groups with six rats in each group. Group I comprises normal healthy rats remains untreated for 90 days;Group II comprises of high cholesterol diet induced atherosclerotic rats for 90 days; Group III, IV, and V comprise of high cholesterol diet induced atherosclerosis and were administered extract from pericarp of mangosteen orally (200mg/kg; 400 mg/kg, 800 mg/kg) daily for 90 days. After 90 days treatment, rats were decapitated and blood was sample collected from the aorta for determination level of TNF-α and IL-1 by ELISA. Aorta was taken to observe the expression of NF-kB, ICAM-1, and IL-6 using immunohistochemical techniques. Results. Oral administration (800 mg/kg) of the extract from pericarp of mangosteen significantly decreased the level of TNF-α, and IL-1 and also reduced the expression of NF-kB, ICAM-1, and IL-6. Conclusion. These results suggest that the extract from pericarp of mangosteen (Garcinia mangostana Linn)possess anti-inflammatory actions by reducing level of TNF-α, IL-1, and also reduce the expression of NF-kB, ICAM-1, and IL-6 that prevent the development of atherosclerosis.