INDUKSI TUNAS MIKRO TIGA VARIETAS BAWANG MERAH (Allium ascalonicum L.) UNGGUL JAWA TIMUR DENGAN MENGUNAKAN IAA DAN BAP SECARA IN VITRO
| Main Author: | Purnomo, Budi |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
http://eprints.umm.ac.id/38229/1/PENDAHULUAN.pdf http://eprints.umm.ac.id/38229/2/BAB%20I.pdf http://eprints.umm.ac.id/38229/3/BAB%20II.pdf http://eprints.umm.ac.id/38229/4/BAB%20III.pdf http://eprints.umm.ac.id/38229/5/BAB%20IV.pdf http://eprints.umm.ac.id/38229/6/BAB%20V.pdf http://eprints.umm.ac.id/38229/7/LAMPIRAN.pdf http://eprints.umm.ac.id/38229/ |
Daftar Isi:
- Indonesian Red Onion production in 2013 reached 958,595 tons, with East Java producing 240,911 tons. Seed propagation that still uses conventional methods, which requires using 1 ton / ha. In Vitro propagation is a technology that is able to produce seeds quickly and in large quantities. IAA and BAP are growth regulating substances that are able to succeed in Vitro culture on explant growth. The study was conducted in February 2015 until May 2015. The experiment was conducted using factorial randomized block design (RBD) with two factors. The first factor was the Onion Varieties (V) which consisted of: V1: Bauji variety, V2: Philipine variety, V3: Thailan variety, and the second factor was the combination of IAA and BAP growth regulators (8), namely A1: 0, 1 ppm IAA + 1 ppm BAP; A2: 0.5 ppm IAA + 1 ppm BAP; A3: 1 ppm IAA + 1 ppm BAP; A4: 1.5 ppm IAA + 1 ppm BAP, A5: 0.1 ppm IAA + 2 ppm BAP; A6: 0.5 ppm IAA + 2 ppm BAP; A7: 1 ppm IAA + 2 ppm BAP; A8: 1.5 ppm IAA + 2 ppm BAP. Observation variables included: number of shoots (fruit), number of roots, number of leaves, when buds appeared (HST), when roots appeared (HST), sprouted explants (%), rooted explants (%), chlorophyll levels (mg / ml), the number of stomata (fruit), and the wet weight of leaves (g). Data were analyzed by F test at 5% level and 1% level and Duncan advanced test 5%. Based on the results of the study, showed an unreal interaction between red onion varieties and a combination of IAA and BAP, on all observational parameters. The differences in the types of varieties used have different effects on the induction of onion shoots, the philiphin variety has the ability to produce roots, leaves, and shoots on explants, while the Bauhji and Thailan varieties have the ability to produce shoots. The shoot induction on the growth of the number of roots and when the roots appeared was influenced by the concentration of the increase in the concentration of IAA 0.1 ppm to 1.0 ppm, and then decreased the concentration of 1.5 ppm. Increased BAP concentration of 2 ppm inhibits root growth, but is able to accelerate when the roots appear