ISOLASI DAN UJI MIKROBA ANTAGONIS POTENSIAL DARI RHIZOSFIR TANAMAN APEL (Malus sylvestris Mill) TERHADAP PATOGEN EMBUN TEPUNG DAN BERCAK DAUN SECARA IN VITRO
| Main Author: | Dwi Cahyanto, Dedy |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
, 2009
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| Subjects: | |
| Online Access: |
http://eprints.umm.ac.id/1913/1/ISOLASI_DAN_UJI_MIKROBA_ANTAGONIS_POTENSIAL_DARI_RHIZOSFIR.pdf http://eprints.umm.ac.id/1913/ |
Daftar Isi:
- Cost of the apple production that’s keep increasing because pesticide price of disease control was more expensive. The microbe isolation of rhizosfir can be used to overcome pathogen as cause of disease of flour dew and leaf spotted at the apple leaf by antagonist test. The ability of resistivity was value of antagonist ability to pressure the growth of pathogen as cause of flour dew and leaf spotted disease. The research objective was to find out microbe antagonist rhizosfir that can control pathogen of flour dew and leaf spotted disease at the apple. Isolation in this research was conducted at flour dew and leaf spotted disease as pathogen cause of disease. Isolation of rhizosfir uses dilution method as anthagonist microbe. The research sample was taken from Batu town East Java. Microbe as result of isolation was tested by test phases: (1) purification of microbe: (1.a) purification of leaf spotted pathogen and flour dew by observed large of microbe surface during for 7 days, (1.b) purification of antagonist microbe as many microbe gain from rhizosfir, (2) antagonistic test: (2.a) test of antagonist microbe by flour dew pathogen, (2.b) test of antagonist microbe by leaf spotted pathogen, (3) calculation of antagonist resistivity toward flour dew and leaf spotted pathogen on the seventh days after inoculation. Based on calculation, can be concluded that: (1) Gain 2 pathogen as cause of flour dew disease (Podoshaera leucotricha) and leaf spotted (Marssonina coronaria J.J. Davis), (2) Gain 10 types of antagonist microbe in Batu town from root land of healthy and unhealthy plant production centre of the apple consist of 8 mushroom isolate and 2 bacteria isolate, (3) Level of antagonist isolate was able to hamper the growth of pathogen at antagonist test with flour dew pathogen (Podoshaera leucotricha) from resistivity test start from the biggest A7, A1, A4, A2, A3, A10, A9, A8, A6 and A5 by level of resistivity 79,91%, 72,09%, 69,55%, 56,22%, 53,34%, 39,47%, 39,30%, 18,28% and 14,68%, (4) Level of antagonist isolate was able to hamper the growth of pathogen at antagonist test with leaf spotted pathogen (Marssonina coronaria JJ. Davis) from resistivity test start from the biggest A1, A2, A7, A4, A5, A10, A8, A9, A3, and A6 by resistivity 87,16%, 86,67%, 86,51%, 84,39%, 79,16%, 70,91%, 69,98%, 68,39% and 65,94%.