Effect of Eucheuma cottoni seewead extract on malondialdehid level and spermatozoa of white rat (Rattus norvegicus L.) that induced by natrium nitrite
| Main Authors: | Wirastuti, Dessy Sukma, Sukmaningsih, A S S A, Sudirga, Sang Ketut |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana
, 2022
|
| Online Access: |
https://ojs.unud.ac.id/index.php/bio/article/view/73223 https://ojs.unud.ac.id/index.php/bio/article/view/73223/44812 |
Daftar Isi:
- Processed meat products are types of food that are easily damaged. Damaged to meat products due to the presence of microorganisms grew. To overcome this, meat processing companies have added a lot of food additives (BTP), namely sodium nitrite (NaNO2). Excess nitrite accumulation in the body can have an effect on the reproductive system. Research conducted by Pavlova et al. (2017) found that there was a decrease in epididymal weight and a reduction in the sperm count of rats exposed to high NaNO2. Eucheuma cottoni seaweed contains antioxidants that can neutralize the oxidative properties caused by NaNO2. The purpose of this study is know the effect of Eucheuma Cottoni seewead extract on Malondialdehid Level and Spermatozoa of White Rat (Rattus Norvegicus L.) that induced by NaNO2. The design used was a completely randomized design (CRD) consisting of negatif control treatment (K-), positive control treatment (+), dose treatment 1 (P1), dose treatment 2 (P2) and dose treatment 3 (P3). The negative control treatment (K-) was given distilled water while the positive control treatment was given sodium nitrite at a dose of 22.5 mg / kg BW. Treatment doses 1,2 and 3 were given a combination method of sodium nitrite and seaweed extract at a dose of 150 mg / kgBB / day (P1), 300 mg / kgBB / day (P2), and 450 mg / kgBB / day (P3). Observations were made by observing the levels of MDA (Malondialdehyde) in serum and sperm quality namely integrity of the membrane, viability, motility and morphology of spermatozoa male white rats. Data analysis was carried out through the One Way Anova statistical test and continued with the Duncan test. The results showed that seaweed extract dose of 150 mg/kgBW significantly could decrease MDA (Malondialdehyde) levels, increased viability, motility integrity of the membrane and morphology of spermatozoa.
- Produk daging olahan adalah salah satu jenis pangan yang mudah mengalami kerusakan. Kerusakan pada produk daging olahan diakibatkan karena adanya pertumbuhan mikroorganisme. Menanggulangi hal tersebut, perusahaan pengolahan daging banyak menambahkan bahan tambahan pangan (BTP) yaitu natrium nitrit (NaNO2). Akumulasi nitrit yang berlebih dalam tubuh dapat menimbulkan efek yang merugikan pada sistem reproduksi. Penelitian oleh Pavlova et al. (2017) menemukan terjadi penurunan berat epididimis dan pengurangan jumlah spermatoa tikus yang diberi paparan NaNO2 tinggi. Rumput laut Eucheuma cottoni mengandung antioksidan yang dapat menetralkan sifat oksidatif yang ditimbulkan oleh NaNO2. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak rumput laut Eucheuma cottoni terhadap kadar Malondialdehid (MDA) dan spermatozoa pada tikus putih (Rattus norvegicus L.) jantan yang diinduksi NaNO2. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari kontrol negatif (K-), kontrol positif (K+), perlakuan dosis 1 (P1), perlakuan dosis 2 (P2) dan perlakuan 3 (P3). Kontrol negatif (K-) diberikan aquades sedangkan kontrol positif diberikan natrium nitrit dosis 22,5 mg/kg BB. Perlakuan dosis 1,2 dan 3 diberikan metode kombinasi natrium nitrit dan ekstrak rumput laut dosis 150 mg/kgBB/hari (P1), 300 mg/kgBB/hari (P2), dan 450 mg/kgBB/hari (P3). Pemberian perlakuan dilakukan selama 45 hari. Adapun variabel penelitian terdiri dari kadar MDA (Malondialdehid) pada serum dan kualitas spermatozoa meliputi integritas membran, viabilitas, motilitas dan morfologi spermatozoa dari tikus putih jantan. Analisis data dilakukan melalui uji statistik One Way Anova dan dilanjutkan dengan uji Duncan. . Hasil penelitian menunjukkan ekstrak rumput laut dosis 150 mg/kgBB mampu menurunkan kadar MDA (Malondialdehid), meningkatkan motilitas, viabilitas, integritas membran dan morfologi spermatozoa secara signifikan.