ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI
| Main Authors: | Setiawati, Agustina, Yuliani, Sri Hartati, Istyastono, Enade Perdana, Gani, Michael Rahardja, Veronica, Evy Fenny, Putri, Dina Christin Ayuning, Putra, Reza Eka, Putra, David Chandra, Kurniawan, Agnes Mutiara |
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| Format: | Article info application/pdf |
| Bahasa: | eng |
| Terbitan: |
Sanata Dharma University
, 2014
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| Online Access: |
http://e-journal.usd.ac.id/index.php/JFSK/article/view/80 http://e-journal.usd.ac.id/index.php/JFSK/article/view/80/68 |
Daftar Isi:
- Abstract: Rapid and simple quantitative analysis isoflavones tempe using densitometric TLC has been done. The mobile phase of the system was chloroform: methanol: ethylacetate (45: 5: 0.75). Thin layer chromatography was performed on aluminium TLC plates.Ascending distance of 1 μL sample was performanced 10 cm. Then the plate was scanned at 261 nm. A linear relationship obtained at 0.08 - 2 μg/spot with r= 0.9986. The LOD and LOQ of isoflavone were 0.014 μg/spot and 0.048 μg/spot. Genistein contained in tempe was 0.151± 0.005 % b/b.Keywords: tempe, isoflavon, TLC