Cytotoxicity Assay Using Brine Shrimp Lethality Test on Collagen Chitosan Wond Dressing Sterilized by Ultraviolet Light
| Main Authors: | Andini, Ary, Prayekti, Endah, Wulandari, Devyana Dyah, Nidianti, Ersalina |
|---|---|
| Format: | Article PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
UNUSA Press
, 2020
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| Subjects: | |
| Online Access: |
http://repository.unusa.ac.id/6231/1/Cytotoxicity%20Assay%20Using%20Brine%20Shrimp%20Lethality%20Test%20on%20Collagen%20Chitosan%20Wond%20Dressing%20Sterilized%20by%20Ultraviolet%20Light.pdf http://repository.unusa.ac.id/6231/2/peer%20review.pdf http://repository.unusa.ac.id/6231/3/turnitin.pdf http://repository.unusa.ac.id/6231/ https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1467 https://doi.org/10.33086/ijmlst.v2i1.1467 |
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6231 |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>http://repository.unusa.ac.id/6231/</relation><title>Cytotoxicity Assay Using Brine Shrimp Lethality Test on Collagen Chitosan Wond Dressing Sterilized by Ultraviolet Light</title><creator>Andini, Ary</creator><creator>Prayekti, Endah</creator><creator>Wulandari, Devyana Dyah</creator><creator>Nidianti, Ersalina</creator><subject>RS Pharmacy and materia medica</subject><description>Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 >1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50 >1000.</description><publisher>UNUSA Press</publisher><date>2020-04</date><type>Journal:Article</type><type>PeerReview:PeerReviewed</type><type>File:application/pdf</type><language>eng</language><identifier>http://repository.unusa.ac.id/6231/1/Cytotoxicity%20Assay%20Using%20Brine%20Shrimp%20Lethality%20Test%20on%20Collagen%20Chitosan%20Wond%20Dressing%20Sterilized%20by%20Ultraviolet%20Light.pdf</identifier><type>File:application/pdf</type><language>eng</language><identifier>http://repository.unusa.ac.id/6231/2/peer%20review.pdf</identifier><type>File:application/pdf</type><language>eng</language><identifier>http://repository.unusa.ac.id/6231/3/turnitin.pdf</identifier><identifier> Andini, Ary and Prayekti, Endah and Wulandari, Devyana Dyah and Nidianti, Ersalina (2020) Cytotoxicity Assay Using Brine Shrimp Lethality Test on Collagen Chitosan Wond Dressing Sterilized by Ultraviolet Light. Indonesian Journal of Medical Laboratory Science and Tehnology, 2 (1). pp. 21-26. ISSN 2656-9825 </identifier><relation>https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1467</relation><relation>https://doi.org/10.33086/ijmlst.v2i1.1467</relation><recordID>6231</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal PeerReview:PeerReviewed PeerReview File:application/pdf File |
| author |
Andini, Ary Prayekti, Endah Wulandari, Devyana Dyah Nidianti, Ersalina |
| title |
Cytotoxicity Assay Using Brine Shrimp Lethality Test on Collagen Chitosan Wond Dressing Sterilized by Ultraviolet Light |
| publisher |
UNUSA Press |
| publishDate |
2020 |
| topic |
RS Pharmacy and materia medica |
| url |
http://repository.unusa.ac.id/6231/1/Cytotoxicity%20Assay%20Using%20Brine%20Shrimp%20Lethality%20Test%20on%20Collagen%20Chitosan%20Wond%20Dressing%20Sterilized%20by%20Ultraviolet%20Light.pdf http://repository.unusa.ac.id/6231/2/peer%20review.pdf http://repository.unusa.ac.id/6231/3/turnitin.pdf http://repository.unusa.ac.id/6231/ https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1467 https://doi.org/10.33086/ijmlst.v2i1.1467 |
| contents |
Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 >1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50 >1000. |
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Universitas Nahdlatul Ulama Surabaya (UNUSA) |
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Professional Nurses/Keperawatan Profesional Obstetric, Midwifery/Obstetri, Ilmu Kandungan, Ilmu Kebidanan Medicine and Health/Ilmu Kedokteran, Ilmu Pengobatan dan Ilmu Kesehatan |
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