The Assessment Of Different Level Of Tissue Inhibitor Of Metalloproteinase (TIMP)-1 From Periodontal Fluid Among Smokers
| Main Authors: | Azhary, Mulkan, Amin, Muhammad, Soetjipto, ., Mulyadi, ., Sunnati, . |
|---|---|
| Format: | Article PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
Asian Pacific Society of Respirology
, 2017
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| Subjects: | |
| Online Access: |
http://repository.unusa.ac.id/6137/4/The%20Assessment%20Of%20Different%20Level%20Of%20Tissue%20Inhibitor%20Of%20Metalloproteinase%20%28TIMP%29-1%20From%20Periodontal%20Fluid%20Among%20Smokers.pdf http://repository.unusa.ac.id/6137/19/peer%20review.pdf http://repository.unusa.ac.id/6137/3/turnitin%20mulyadi.pdf http://repository.unusa.ac.id/6137/ https://onlinelibrary.wiley.com/doi/full/10.1111/resp.13207_229 |
Daftar Isi:
- Background and Aim: The periodontium is vulnerably exposed during smoking leading to the extracellular matrix (ECM) destruction due to active activity of proteases. Lowly balanced level of TIMP-1 induces the progression of tissue destruction. This study aimed to assess the differ- ence level of TIMP-1 from gingival crevicular fluid (GCF) in order to evaluate the presumed ECM destruction among smokers, especially ex smokers suffering from COPD. Methods: The study included 30 male smokers, of whom 15 were ex smokers suffering from COPD. They underwent the physical examination and spirometry. The criteria of COPD based on post bronchodilator FEV1/FVC < 70% with FEV1% predicted <50 %. Then, the gingival crevicular fluid (GCF) was absorbed by inserting a small piece of filter paper into the gingival sulci. The measurement of TIMP-1 level from GCF used the ELISA method applying R&D system. Conclusion: The level of TIMP-1 was elevated among healthy smokers balancing the increase of protease which is naturally essential to reduce the ECM destruction leading to progressive periodontium destruction. The higher level of TIMP-1 was correlated with the smoking pack year which was slightly higher among healthy smokers. The periodontium is more likely sensitive against the continuous smoking exposure. The emphysematous lung parenchyma is likely evaluated through the level of TIMP-1 from sputum, then compared to that from periodontium.