Morphological, Physiological and Molecular Characteristics of Tolerant Sugarcane to Waterlogging Stress
| Main Authors: | Wibisono, Viki Bayu , Avivi, Sholeh , Ubaidillah, Mohammad , Sri Hartatik |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia
, 2022
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| Online Access: |
http://journal.ipb.ac.id/index.php/jurnalagronomi/article/view/40760 http://journal.ipb.ac.id/index.php/jurnalagronomi/article/view/40760/24034 |
Daftar Isi:
- Cekaman genangan menyebabkan penurunan hasil 15-45% pada tanaman tebu. Penelitian ini bertujuan untuk mengetahui karakteristik morfologi, fisiologi, dan molekuler tanaman tebu toleran terhadap cekaman genangan. Percobaan dilaksanakan di Laboratorium Program Studi Agroteknologi, Fakultas Pertanian, Universitas Jember, Jawa Timur pada bulan September 2021-Februari 2022. Rancangan percobaan menggunakan rancangan acak kelompok (RAK) faktorial. Faktor pertama yaitu genotipe tebu dengan enam genotipe (Bululawang, PS 862, Cening, PS 881, M3, M4). Faktor kedua yaitu periode penggenangan dengan tiga taraf (30 hari, 60 hari, dan 90 hari). Faktor ketiga yaitu perlakuan cekaman genangan (tanpa cekaman genangan dan dengan cekaman genangan). Variabel pengamatan meliputi tinggi tanaman, luas permukaan daun, diameter batang, bobot segar akar, jaringan aerenkim, klorofil total, kerapatan stomata, kandungan H2O2 dan ekspresi gen antioksidan. Hasil penelitian menunjukkan bahwa cekaman genangan berdampak pada pembentukan jaringan aerenkim, penurunan bobot segar akar, perubahan tinggi tanaman, penurunan luas area daun, penurunan klorofil total, penurunan kerapatan stomata, peningkatan kandungan hidrogen peroksida dan ekspresi gen antioksidan. Genotipe Cening menunjukkan respon persentase penurunan yang rendah dibandingkan dengan kontrol tanpa cekaman pada variabel morfologi, fisiologis dan ekspresi gen antioksidan yang lebih tinggi. Kata kunci: antioksidan, banjir, ketahanan, reactive oxygen species, Cening
- Waterlogging stress reduced sugarcane yield by 15-45%. This study aimed to determine the morphological, physiological, and molecular response of sugarcane under waterlogging stress. This research was conducted from October 2021 to January 2022 at the Agrotechnology Departement Laboratory, Faculty of Agriculture, Jember University, East Java. The experiment was arranged using a randomized block factorial design. The first factor was the genotype of sugarcane with 6 levels (Bululawang, PS 862, Cening, PS 881, M3, M4). The second factor was the waterlogging period with 3 levels (30 DAT, 60 DAT, and 90 DAT). The third factor was waterlogging treatment (without waterlogging treatment and with waterlogging treatment). Several growth characteristics such as fresh root weight, stem diameter, plant height, leaf area surface, aerenchyma tissue, total chlorophyll, stomata density, H2O2, and antioxidant gene expression were investigated. The results showed that waterlogging stress had an impact on the formation of aerenchyma tissue, plant height, fresh root weight, leaf area, total chlorophyll, stomata density, hydrogen peroxide, and expression of antioxidant gene. Cening genotype showed the best response in terms of low decreasing percentage to morphological, physiological, and high antioxiant gene expression. Keywords: antioxidant, flooding, resistance, reactive oxygen species, Cening