Isolation of Mercury-Resistant Endophytic and Rhizosphere Microorganisms from Grasses in Abandoned Gold Mining Area
| Main Authors: | Reni Ustiatik, Yulia Nuraini, Suharjono, Eko Handayanto |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia
, 2021
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| Online Access: |
http://journal.ipb.ac.id/index.php/jurnalagronomi/article/view/32356 http://journal.ipb.ac.id/index.php/jurnalagronomi/article/view/32356/21585 |
Daftar Isi:
- There were about 900 hotspots of artisanal and small scale gold mining (ASGM) in Indonesia that recovered gold through amalgamation and cyanidation techniques. Amalgamation technique causes mercury (Hg) pollution to the soil. This study was a preliminary study that aimed to isolate Hg-resistant endophytic and rhizosphere microorganisms from pioneer grasses in the Hg-polluted soil. The most potential microorganism will be used for Hg phytoremediation in the future study. Pioneer grasses were collected from the abandoned gold mining area in Central Lombok Regency, West Nusa Tenggara. Total microorganisms were counted using Colony Forming Unit (CFU) or Standard Plate Count. The microorganism colony was characterized based on morphological characteristics. Hg-resistant endophytic and rhizosphere microorganisms were successfully isolated from pioneer grass (Cynodon dactylon and Eleusine indica) in the study site. The colonies of rhizosphere microorganisms were diverse morphologically compared to endophytic microorganisms based on the number of isolated microorganisms, 20 isolates and 17 isolates, respectively. The density of rhizosphere microorganisms was higher (96%) than endophytic microorganisms (4%). The density of rhizosphere bacteria and fungi were 47x103 and 2x103 CFU g-1, respectively. However, the density of endophytic bacteria and fungi were only 2x103 and 1x103 CFU g-1, respectively. Keywords: endophytic microorganism, Hg-resistant, microorganism density, rhizosphere microorganism
- Terdapat sekitar 900 titik pertambangan emas skala kecil (PESK) di Indonesia yang memperoleh emas melalui teknik amalgamasi dan sianidasi. Teknik amalgamasi menyebabkan pencemaran merkuri (Hg) di tanah. Penelitian ini merupakan penelitian pendahuluan (preliminary study) yang bertujuan untuk mengisolasi mikroorganisme endofit dan rhizosfer resisten Hg dari rumput pionir yang tumbuh di tanah yang tercemar Hg. Mikroorganisme paling berpotensi akan diaplikasikan pada fitoremediasi merkuri di penelitian selanjutnya. Sampel rumput pionir diambil dari lahan pertanian bekas kawasan pertambangan emas dengan teknik amalgamasi di Desa Bonjeruk, Kecamatan Jonggat, Kabupaten Lombok Tengah, Nusa Tenggara Barat. Total mikroorganisme dihitung menggunakan Colony Forming Unit (CFU) atau Standard Plate Count. Koloni mikroorganisme dikarakterisasi berdasarkan ciri morfologi. Mikroorganisme endofit dan rizosfer yang resisten Hg berhasil diisolasi dari rumput pionir (Cynodon dactylon dan Eleusine indica) di lokasi penelitian. Koloni mikroorganisme rizosfer sangat beragam secara morfologi dibandingkan dengan mikroorganisme endofit berdasarkan jumlah mikroorganisme terisolasi, berturut-turut 20 isolat dan 17 isolat. Kepadatan mikroorganisme rizosfer lebih tinggi (96%) dibandingkan mikroorganisme endofit (4%). Kepadatan bakteri dan jamur rizosfer masing-masing adalah 47x103 dan 2x103 CFU g-1 sedangkan kepadatan bakteri endofit dan jamur masing-masing hanya 2x103 dan 1x103 CFU g-1.