Sintesis Heptapeptida Linear (H-Tyr-Asp-Pro-Ala-Pro-Pro-Pro-Oh) dengan Menggunakan Dic/Oksima sebagai Reagen Pengkopling
| Main Authors: | Maharani, Rani, Yanti, Eka Fitri |
|---|---|
| Other Authors: | Fakultas Sains dan Teknologi UIN Alauddin Makassar |
| Format: | Article info application/pdf eBookSection |
| Bahasa: | eng |
| Terbitan: |
Study Program of Chemistry - Alauddin State Islamic University of Makassar
, 2016
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| Subjects: | |
| Online Access: |
http://journal.uin-alauddin.ac.id/index.php/al-kimia/article/view/1449 http://journal.uin-alauddin.ac.id/index.php/al-kimia/article/view/1449/1477 |
Daftar Isi:
- A tetrapeptide, an analog of trypsin-modulating oostatic factor (TMOF), has been synthesised by using a method of solid-phase peptide synthesis on 2-chlorotrityl chloride resin. Fmoc strategy was applied on the synthesis. The formation of peptide bond was facilitated by DIC/oxime as coupling reagent. After all of amino acids were attached on the resin, tetrapeptidyl resin was added by a mixture of TFA:water:EDT(90:5:5) in dichloromethane to cleave the peptide.Crude peptide was purified by reverse-phase column chromatography and the purified peptide was analysed by TOF ES-MS spectroscopy.