The Role of Red Fruit (Pandanus conoideus Lam) Oil towards Colorectal Cancer Mice Model
| Main Authors: | Khiong, Khie; Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Adhika, Oeij Anindita; Faculty of Medicine, Universitas Kristen Maranatha, Bandung |
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| Format: | application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Journal of the Indonesian Medical Association
, 2012
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| Online Access: |
http://indonesia.digitaljournals.org/index.php/idnmed/article/view/1300 |
Daftar Isi:
- Introduction: Colorectal cancer (CRC) is a long-term complication of inflammatory bowel disease (IBD) and has become the leading cause of death worlwide. Cyclooxygenase-2 (COX-2) is a key enzyme that produced in IBD and CRC. Therefore, inhibition COX-2 has become a potential mechanism to prevent colorectal carcinogenesis. Red fruit (Pandanus conoideus Lam.) is plant from Papua which contains large amount of antioxidant.Objectives: To examine the effect of red fruit towards clinical score and COX-2 expression in colorectal cancer mice model.Methods: Subjects were divided into four groups: the negative and red fruit control group which were not induced colorectal cancer, positive control and red fruit-treated groups which were induced. Clinical score was observed every two days. At the end of experiment, all mice were sacrificed and the colons were removed and subjected to RT-PCR analysis. COX-2 persentage were measured using RT-PCR method.Results: Clinical score and COX-2 expression of red fruit treated group was significantly decreased compared to the positive control group. There is no significant difference were observed between the negative control group, red fruit control group, and red fruit treatment group. Conclusion: Red fruit oil improves clinical score and decreases COX-2 expression in colorectal cancer mice model.Keywords: IBD, colorectal cancer, COX-2, red fruit