Action Target of Curcumin and PGV-0 on Luteal Cells Steroidogenesis through the Expression of Cytochrome P450scc
| Main Authors: | Purwaningsih, Endang; Faculty of Medicine, Universitas YARSI, Jakarta, Soejono, Sri Kadarsih; Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Dasuki, Djaswadi; Universitas Gadjah Mada, Yogyakarta, Meiyanto, Edy; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta |
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| Format: | eJournal |
| Bahasa: | eng |
| Terbitan: |
Journal of the Indonesian Medical Association
, 2012
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| Online Access: |
http://indonesia.digitaljournals.org/index.php/idnmed/article/view/1229 |
Daftar Isi:
- Introduction: Curcumin and its analog pentagamavuon-0/PGV-0 can inhibit steroidogenesis of luteal cell culture. However, the action site of the curcumin and PGV-0 in luteal cells steroidogenesis is still unknown.Objectives: To analyze the action site of curcumin and PGV-0 in luteal cells steroidogenesis through its influences on the expression of cytochrome P450scc enzyme.Methods: PMSG-induced corpus luteums of Sprague Dawley rat were used as the subjects. Curcumin and PGV-0 with a dosage of 100 μM was given shortly after the stimulation of LH and/or PGF2a. Immunohistochemistry staining was done to assess the expression of cytochrome P450scc.Results: LH stimulation (50 ng/ml) increased the expression of cytochrome P450scc significantly (p<0.05) compared with the control group. In the other hand, PGF2a (0.56 μM) inhibited the expression of cytochrome P450scc significantly. Curcumin (100 μM) and PGV-0 (100 μM) inhibited the expression of cytochrome P450scc in the control group and groups stimulated by LH and/or PGF2a.Conclusion: Curcumin and PGV-0 inhibit the upstream transduction signal of cytochrome P450scc in the steroidogenesis of luteal cell culture. J Indon Med Assoc. 2012;62:136-41.Keywords: P450scc, steroidogenesis, transduction signal