The Effectiveness of Typhonium flagelliforme Tuber Extract of Diclorometanolic fraction on The Inhibition of Proliferation of MCF-7 Human Breast Cancer Cell-Line
| Main Authors: | Putra, Agung; Faculty of Medicine Universitas Islam Sultan Agung, Semarang, Tjahjono, Tjahjono; Faculty of Medicine Universitas Diponegoro, Semarang, Winarto, Winarto; Faculty of Medicine Universitas Diponegoro, Semarang |
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| Format: | application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Journal of the Indonesian Medical Association
, 2012
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| Online Access: |
http://indonesia.digitaljournals.org/index.php/idnmed/article/view/1120 |
Daftar Isi:
- Introduction: Breast cancer has been shown to have the ability to survive by proliferation.Typhonium flagelliforme (Lodd), known as keladi tikus has ability of anti-proliferation on themurine P388 leukemia cell and NCI-H23 non-small cell lung carcinoma cell line, depend on thepolarity of solvent and structure of cancer cell. The aim of this study was to compare the effectivenessof tuber and leave extract of Typhonium flagelliforme to inhibit the cell proliferation of MCF-7 breast cancer cell line.Methods: in-vitro experimental study on the MCF-7 cell “Post test control group only design” the cell lines were divided into 49 groups that include the control group, 3 groups treated with DMC, ethanolic, hexanolic extract of Typhonium flagelliforme and 3 groups treated withDCM, ethanolic , and hexanolic extract of the leave of Typhonium flagelliforme at concentrationof 1000, 500, 250, 125, 62,5, 31,25, 15,62, 7,81μg/m. Cell proliferation was estimated byMTT assay, log probit was used for the statistical analysis.Results: DMC extract of the tuber of Typhonium flagelliforme inhibit the cell proliferation at IC5o= 63.08 μg/mL (nilai p<0.001), and the DMC extract of the leave of Typhonium flagelliformeinhibit cell proliferation at IC5o = 68.65 μg/mL.Conclusion: The tuber extract of Typhonium flagelliforme is a better potent inhibitor of proliferationin the MCF-7 breast cancer cell line compared to the leave extract. J Indon Med Assoc.2012;62:10-5.Keywords: antiproliferation, Typhonium flagelliforme, MTT Method, IC50