The influence of elicitor Cu2+ and Fe 2+ to asiaticoside level of pennywort (Centella asiatica L. urban) callus
| Main Authors: | Savitri, Evika Sandi, Dwi Khoriroh, Feni, Lugiati Sholikhah, Luluk |
|---|---|
| Format: | Proceeding PeerReviewed Book |
| Bahasa: | eng |
| Subjects: | |
| Online Access: |
http://repository.uin-malang.ac.id/4417/2/4417.pdf http://repository.uin-malang.ac.id/4417/ |
Daftar Isi:
- Pennywort (Centella asiatica L. Urban is a medicinal plant used in traditional herbal medicine industry in Indonesia. Production of bioactive compounds through tissue culture can be enhanced by elicitation. This study was an experimental study using completely randomized design.The treatment used is various concentration of metal ions Fe2+ on callus subculture medium namely 0, 90, 100, and 110 mM. with 4 replications to obtain 16 trials. Cu2+ ion concentration treatments used consisted of 4 levels: Cu0 = control (0 mM), Cu1=30 mM , Cu2 = 35 mM, Cu3 =40 mM with 4 replications to obtain 16 trials Elicitor Cu2+ with 40μM concentration resulted in the high levels production of secondary metabolites asiaticoside 4.1595 g / 100g. Elicitor Fe2+ with a concentration of 100 mM asiaticoside produce secondary metabolite 3.836 g / 100g. Elicitor Cu2 + produces higher levels asiaticoside compared with Fe2+.