Antibodies against Shigella flexneri adhesion molecule outer membrane protein (OMP) can cross-react with OMPs of some Shigella species
| Main Authors: | Milliana, Alvi, Noorhamdani, A.S., Poeranto, Sri, Handono, Kusworini, Prawiro, Sumarno Reto, Fitrianingsih, Avin Ainur, Rachma, Lailia Nur |
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| Format: | Journal PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
, 2017
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| Online Access: |
http://repository.uin-malang.ac.id/3510/1/3510.pdf http://repository.uin-malang.ac.id/3510/ http://doi.org/10.4314/tjpr.v16i2.2 |
Daftar Isi:
- Purpose: To identify a Shigella flexneri hemagglutinin outer membrane protein (OMP) as an adhesion factor and examine its ability to cross-react with the OMPs of other Shigella species. Methods: OMP was isolated from the bacterium S. flexneri after shaving the pili using a pili bacterial cutter in a solution of 0.5 % n-octyl-β-D-glucopyranoside. Hemagglutination was evaluated using mice erythrocytes. The molecular weight (MW) of the OMPs was profiled by SDS-PAGE. Adhesion molecules were identified by calculating the index of adhesion. Thereafter, antigen cross-reaction with antibodies was examined by Western blotting. Results: The S. flexneri adhesion molecule OMP has a MW of 28 kDa. An antibody against the 28 kDa S. flexneri OMP adhesion molecule cross-reacted with different MW OMPs from other Shigella species. Conclusion: This study showed that the 28 kDa S. flexneri OMP is a hemagglutinin adhesion protein, and antibodies against it can cross-react with the OMPs of other Shigella species.