Comparison the anticancer effect of extract and fraction calotropis gigantea radix on human colon cancer WiDr and breast cancer t47d cell lines
| Main Authors: | Mutiah, Roihatul, Widyawaruyanti, Aty, Sukardiman, Sukardiman |
|---|---|
| Format: | Proceeding PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
, 2015
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| Subjects: | |
| Online Access: |
http://repository.uin-malang.ac.id/1994/2/1994.pdf http://repository.uin-malang.ac.id/1994/ http://greentech.uin-malang.ac.id/ |
Daftar Isi:
- The anticancer effect of extract And fraction Calotropis gigantea radix on human colon cancer WiDr And Breast Cancer T47D Cell Lines have been evaluated. The ethanolic extract was fractionated gradually with dicloromethane (DCM), ethyl acetate (EA) and butanol (BuOH) to yield four fractions including DCM fraction, EA fraction, BuOH fraction and water fraction. The anticancer effect was performed using MTT method. The IC50 was used to express the anticancer potency. The result showed that extract and fraction of Calotropis gigantea radix have much more potent to colon cancer WiDr than breast cancer T47D cell lines. IC50 on WiDr cell lines were etanol extract (44,2 μg/ml), DCM fraction (14,92 μg/ml), EA fraction (1,25 μg/ml), BuOH fraction (1,12 μg/ml) dan water fraction (>1000 μg/ml). IC50 on T47D cell lines were etanol extract (89,75 μg/ml), DCM fraction (131,29 μg/ml), EA fraction (55,89 μg/ml), BuOH fraction (96,72 μg/ml), water fraction (>1000 μg/ml). DCM, EA and BuOH fractions are potential to be developed as an anticancer agent in colon cancer therapy.