Induction of Somatic Embryos from Leaf and Stem Nodal Section Explants of Potato (Solanum tuberosum L.)
| Main Authors: | Iriawati, I.; School of Life Sciences & Technology Institut Teknologi Bandung, Aprilianty, Fitri; Biology Department of School of Life Sciences and Technology, Institut Teknologi Bandung Jl. Ganesha 10 Bandung 40132, Indonesia |
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| Other Authors: | This research was supported by grant in aid from Indofood Riset Nugraha 2012 program. PT.Indofood Sukses Makmur, Tbk. |
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
ITB Journal Publisher, LPPM ITB
, 2014
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| Subjects: | |
| Online Access: |
http://journals.itb.ac.id/index.php/jmfs/article/view/611 http://journals.itb.ac.id/index.php/jmfs/article/view/611/769 http://journals.itb.ac.id/index.php/jmfs/article/downloadSuppFile/611/73 http://journals.itb.ac.id/index.php/jmfs/article/downloadSuppFile/611/74 |
Daftar Isi:
- Somatic embryogenesis can be used as an alternative method to obtain high-quality potato seedlings. This research was conducted in order to evaluate: the potential of different types of explants to produce somatic mbryos;combinations of 2,4-D and BAP for somatic embryo induction; and the ability of BAP to act as growth regulator for the somatic embryo maturation process.Young leaf and stem nodal sections were cultured in MS media supplemented with 2,4-D and BAP. The developing calli were then transferred into media containing several concentrations of 2,4-D and BAP. The embryogenic calli were then transferred into media containing 1-5 μM BAP. The results show that MS media containing 2,5 μM 2,4-D + 5 μM BAP and μM 2,4-D + 0.5 μM BAP were the most suitable for inducing somatic embryos from leaf explants and stem nodal section explants, respectively. The somatic embryos were well-developed in the MS media supplemented with 5 μM BAP. The stem nodal section explants were able to produce a higher percentage of globular, heart and torpedo stage embryos compared with the leaf explants. Based on this research, stem nodal section explants have a high potential for producing somatic embryos induced in medium containing 1 μM 2,4-D + 0.5 μM BAP.