Isolation and Characterization of Phytase-Producing thermophilic Bacteria from Sulili Hot Springs in South Sulawesi
Daftar Isi:
- This study aimed to isolate, select and identify phytase-producing thermophilic bacteria from Sulili hot springs in the district Pinrang South Sulawesi, Indonesia. water and sediment samples were obtained from Sulili hot springs the district Pinrang, South Sulawesi. Isolation was done using Luria Bertani (LB) medium at 60°C for 1 x 24 hours. Twelve isolates of thermophilic bacteria have been obtained, five of them have the ability to produce phytase. Indicated by the formation of a clear zone around the colonies grown on medium screening LB + Ca-phytate. Five isolates were then selected, three of whom are selected based isolates widest phytase activity qualitatively. Morphological characteristics and biochemical nature of the three selected isolates have been identified belong to genera of Bacillus. Each has similarities with Bacillus licheniformis, Bacillus coagulans and Bacillus stearothermophillus.