The effect of gamma radiation on microbial content and curcuminoids of curcuma amada roxb.rhizomes
| Main Author: | D.P.Rahayu |
|---|---|
| Format: | Journal |
| Terbitan: |
Center for Informatics and Nuclear Strategic Zone Utilization
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| Subjects: |
Daftar Isi:
- The microbial contamination in the rhizomes of medicinal plants including curcuma amada rhizomes is generally high. this due to the fact that rhizomes are the bottom parts that grow in the soil. based on the regulation of head of the indonesian national agency of drug and food control number HK.00.06.1.52.4011,the limits of microbial contamination in herbal/medicinal plants are 10 cfu/g for the total microbial and 2x10 cfu/g for the total yeast and mold.gamma irradiation is one of the methods to reduce microbial contaminatiion in medicinal plants. in this effectiveness of gamma irradiation in microbial reduction and its effect to curcuminoid contents was determined by irradiating curcuma amada rhizomes at doses of 5 and 10 kGy. the initial contamination in this rhizomes was 8.78x10 cfu/g and 5x10 cfu/g for the total microbial and for the total yeast and mould,respectively. the result indicates that at 5 kGy,the microbial contamination and the mould and yeast contamination were reduced from 8.78x10 cfu/g and 5x10 cfu/g to 1.39x10 cfu/g and under 1x10 cfu/g,respectively. meanwhile the comparison of curcuminoids betweeen the irradiated and non irradiated samples was performed by HPLC method and was found to actually increase from 0.26% to 0.36% after the 5-kGy irradiation. it can be concluded that an irradiation dose of 5 kGy is effective to reduce the content of microorganisms without lowering curcuminoids. gamma radiation could be used as decontamination method in medicinal plants.