OPTIMIZATION OF EXOPOLYSACCHARIDE PRODUCTION BY Lactobacillus casei AL.15
| Main Authors: | Pinaria, Yeanly Wuena; Universitas Kristen Indonesia Tomohon, Antara, Nyoman Semadi; Faculty of Agricultural Technology, Udayana University, Putra, G.P. Ganda; Faculty of Agricultural Technology, Udayana University, Sujaya, I Nengah; Faculty of Medicine, Udayana University |
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| Other Authors: | Univeritas Udayana Bali |
| Format: | Article info Bachelors application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
BBSPJI Hasil Perkebunan, Mineral Logam, dan Maritim
, 2023
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| Subjects: | |
| Online Access: |
http://ejournal.kemenperin.go.id/bbihp/article/view/8050 http://ejournal.kemenperin.go.id/bbihp/article/view/8050/6327 |
Daftar Isi:
- Exopolysaccharide (EPS) is one of the polysaccharides produced from microorganisms. This polysaccharide is usually produced by lactic acid bacteria and it is widely used for food products and pharmaceutical products. EPS are also very useful for stabilizers, emulsifiers, gelling agents, and have a good ability to bind liquids (water). This study aims to determine the optimization point of EPS growth, based on temperature, time, and the amount of glucose used in selective growth media of Lactobacillus casei (L. casei) AL.15, namely de Mann Rogosa Shape Broth (MRSB) media, using Response Surface Method (RSM) design to obtain optimum conditions that are more appropriate to produce EPS. Optimization of EPS production occurred at level -1; -1; 0, temperature 42 °C, incubation time 48 hours, and the addition of 10% glucose. L. casei AL.15 has a good ability to produce EPS at the temperature, time, and amount of glucose obtained. The result obtained under these conditions is 606.03 mg EPS/litre. This method can be used to produce large amounts of EPS and then these characteristics were analyzed.