Screening of Rhizobacterial Indigenous as Biocontrol Agents Against Bacterial Wilt on Chili Using In Planta Technique
| Main Authors: | Habazar, Trimurti, Yanti, Yulmira, Reflinaldon, Reflinaldon, Arneti, Arneti, Nasution, Chainur Rahman |
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| Other Authors: | Isnansetyo, Alim, Nuringtyas, Tri Rini |
| Format: | BookSection PeerReviewed |
| Terbitan: |
Springer International Publishing AG 2017
, 2017
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| Subjects: | |
| Online Access: |
http://repo.unand.ac.id/8172/ |
Daftar Isi:
- Bacterial wilt caused by Ralstonia solanacearum is one of the severe diseases on chili. Until now this disease is difficult to control, while these bacteria attack the xylem vessel and are unreachable by any bactericide. One group of important biocontrol agents are the plant growth-promoting rhizobacteria (PGPR). The PGPR have also the ability to increase growth and yield of plants. The aim of this research was to obtain rhizobacterial isolates, which have the ability to control bacterial wilt on chili and to increase growth and yield. This method based on in plant selection of enhanced competitive root-colonizing bacteria from soil samples from rhizosphere, healthy chili in endemic area of bacterial wilt diseases (West Sumatera Province, Indonesia). This approach focuses on indirect mechanisms (systemic induced resistance). This technique has the possibility to find new, easy, and cheap biocontrol organisms. We have isolated 42 rhizobacteria from healthy chili’s rhizosphere. Only 17 rhizobacterial isolates could increase seedling growth of chili compared to control plants; the rest of isolates reduced growth of chili seedlings. There were no bacterial wilt symptom on 13 rhizobacterial isolate introduced chilies and lower infection (33.3%) on two rhizobacterial isolate introduced chilies, compared with control plants (100% wilt and die). We have obtained also two rhizobacterial isolates which could control bacterial wilt diseases and increase growth of chili, the isolate RZ.2.1.AG1 as Bacillus cereus strain INACH001 and the isolate RZ.1.3.AP1 as Bacillus subtilis strain BSn5.