Fermentasi Senyawa Bioaktif Dari Jamur Tricoderma Koningii Untuk Mendapatkan Bahan Baku Fungisida Alamiah Baru
| Main Author: | Djamaan, Akmal |
|---|---|
| Format: | Article PeerReviewed Book Lainnya |
| Bahasa: | eng |
| Terbitan: |
Jurusan Farmasi Fakultas MIPA Universitas Andalas
, 2010
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| Subjects: | |
| Online Access: |
http://repo.unand.ac.id/2177/1/Jurnal_02.pdf http://repo.unand.ac.id/2177/2/Jurnal_02.ps http://repo.unand.ac.id/2177/ |
Daftar Isi:
- The objective of this study is to produce bioactive component from trichoderma koningii by fermentation process. The fermentation medium was used combination of corn water extract, sucrose, calcium carbonate, ferrosulphat, cobal chloride, and water up to 100%. The fermentation was conducted at pH 7.0, agitation 180 rpm, temperature 20-25oC for 5 days. Activity of metabolite that produced was determinate toward Sclerotion rolfsii every 24 hours period during the fermentation process. Product of fermentation was extracted by liquid-liquid extraction method using chloroform, aethyl acetate, n-butanol, and water respectively. Each fraction with concentration 0.1; 0.5 and 1.0% w/v were determined toward S. rolfsii. Result showed that the optimum time of fermentation occurred at 72 hour. The medium that produce highest activity is medium-A content of corn water extract 3%, sucrose 3%, calcium carbonate 0.5%, ferrosulphate 0.1% and water up 100%. The n-butanol fraction refeal the highest activity toward S. rolfsii. Keyword : fermentation, Trichoderma koningii and fungiside